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Method for extracting alpha-glucosidase restraining agent effective ingredient from plants

A technology of glucosidase and active ingredients, which is applied in the preparation of Chinese herbal medicines and the fields of chemical and chemical engineering, can solve the problems of no α-glucosidase inhibitors, and achieve the effects of no pollution to the environment, simple process and high purity

Inactive Publication Date: 2008-09-03
TSINGHUA UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In recent years, Cyclocarya paliurus has been found to have a hypoglycemic effect, but there is no research report on the extraction of active ingredients of a-glucosidase inhibitors from it

Method used

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  • Method for extracting alpha-glucosidase restraining agent effective ingredient from plants

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] In the present embodiment, the extraction process and steps of α-glucosidase inhibitor are as follows:

[0015] (1) First, crush 2g of plant leaves; put a 250ml beaker and add 50% ethanol solution at a ratio of 1:40 (g / ml), shake at room temperature for 3h; centrifuge at 8000rpm / min for 20min at room temperature, and harvest the supernatant; plant residues are added 50% ethanol solution, repeat the extraction 3 times; combine the 3 extractions;

[0016] (2) Recover the ethanol solution with a rotary evaporator to obtain 0.72g of solid, add 100ml of hot water for redissolving; add ethyl acetate solution for extraction at a ratio of 1:1, collect 85ml of the aqueous phase; in the harvest solution at a ratio of 1:1 Add n-butanol solution for extraction, collect the water phase part, spin dry to obtain 0.48g solid, 5ml hot water redissolving for use;

[0017] (3) MARC (macroporous adsorption resin) chromatography on the above sample (filler height 35cm, column diameter 2.6c...

Embodiment 2

[0020] The process of the present embodiment 2 is exactly the same as that of the embodiment 1, and its steps are as follows:

[0021] (1) First, crush 2g of plant leaves; put a 250ml beaker and add 50% ethanol solution at a ratio of 1:40 (g / ml), shake at room temperature for 3h; centrifuge at 8000rpm / min for 20min at room temperature, and harvest the supernatant; plant residues are added 50% ethanol solution, repeat the extraction 3 times; combine the 3 extractions;

[0022] (2) Recover the ethanol solution with a rotary evaporator to obtain 0.81g of solid, add 100ml of hot water for redissolving; add ethyl acetate solution for extraction at a ratio of 1:1, collect 89ml of the aqueous phase; in the harvest solution at a ratio of 1:1 Add n-butanol solution for extraction, collect the water phase part, spin dry to obtain 0.52g solid, redissolve in 5ml hot water for use;

[0023] (3) MARC (macroporous adsorption resin) chromatography on the above sample (filler height 35cm, col...

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Abstract

The invention relates to a method for extracting effective ingredient as Alpha-glucosidase inhibitor, which belongs to the fields of Chinese herbal medicine preparation and chemical technology. The method comprises the following steps of: performing liquid extraction with organic solvent to remove ingredients with low Alpha-glucosidase inhibitory activity, and extracting effective ingredients with high Alpha-glucosidase inhibitory activity by using macroporous adsorptive resin and Sephadex LH-20 column chromatography technique. The yield of the inhibitor is 4%. The inventive method has the advantages of simple process, easy operation, high product purity, no harmful substances in product, and no environmental pollution. The obtained plant extract in the invention can be used for developing hypoglycemic drugs and further researching physical structure thereof.

Description

technical field [0001] The invention relates to a method for extracting an α-glucosidase inhibitor active ingredient from plants, and belongs to the technical field of Chinese herbal medicine preparation and chemical engineering. Background technique [0002] Green pennywort is a single genus of Juglandaceae, and it is one of the rare plants in my country. It is a deciduous tree in the form of a deciduous tree, up to 20 meters high. It is mainly distributed in broad-leaved forests on hillsides or mountain nests at an altitude of 400 to 800 meters. Qingqianliu is a unique natural health food resource in China. Green money willow leaves contain germanium, selenium, chromium, vanadium, zinc, iron, calcium and other trace elements. In recent years, it has been found that A. pennywort has a hypoglycemic effect, but there is no research report on extracting the active ingredient of α-glucosidase inhibitor from it. [0003] Alpha-glucosidase (EC 3.2.1.20) is also known as alpha-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/67A61P3/10A61K127/00
Inventor 邱爱东孟凡国胡卫江周海梦
Owner TSINGHUA UNIV
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