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Single sialic acid tetrahexose ganglioside preparation method

A technology of ganglioside sodium and monosialic acid, which is applied to nervous system diseases, medical formulas, medical preparations containing active ingredients, etc., can solve the problems of high cost, large consumption of organic solvents, time-consuming, etc., and achieve improvement Yield, solvent saving and time effects

Inactive Publication Date: 2006-07-12
严家定
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of the above method is that the consumption of organic solvent is large, time-consuming, and both fillers need to be imported, so the cost is high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] 1) Use fresh (or frozen) pig brain to remove the capsule and blood vessels on the surface, add 2 times the volume of cold acetone at room temperature, press and dehydrate for 3 times, filter with multi-layer gauze, and use a pore size of 0.2um Clarify by filtration through a microporous organic membrane, pour out the acetone, grind the dehydrated brain tissue, dry it at 60-80°C (no open flames), and grind it into a fine powder with a dry mill to obtain acetone powder, which is sealed and subpackaged. Save for later use;

[0015] 2) Take 5.0 kg of acetone powder, add 6 kg of extract prepared by adding chloroform: isopropanol: water = 10: 20: 0.1 volume ratio, stir for 1 to 3 hours, filter to obtain clear liquid, and then add 0.2 kg of purified Water is carried out sub-extraction, and supernatant liquid is carried out ultrafiltration with the ultrafiltration membrane that cut-off molecular weight is 3000, obtains ultrafiltrate, extracts through extracting liquid, and extr...

Embodiment 2

[0018] 1) Use fresh (or frozen) bovine brain to remove the capsule and blood vessels on the surface, add 10 times the volume of cold acetone at room temperature, press and dehydrate 6 times, filter with multi-layer gauze, and use a pore size of 1.0um Clarify by filtration through a microporous organic membrane, pour out the acetone, grind the dehydrated brain tissue, dry it at 60-80°C (no open flames), and grind it into a fine powder with a dry mill to obtain acetone powder, which is sealed and subpackaged. Save for later use;

[0019] 2) Take 5.0Kg of acetone powder, add chloroform: isopropanol: water = 50:80:10 volume ratio of the extract solution 50kg and stir for 1 to 3 hours, filter to obtain a clear solution, then add 10kg of purified water to carry out Fractional extraction, the supernatant liquid is ultrafiltered with an ultrafiltration membrane with a molecular weight cut-off of 100,000 to obtain the ultrafiltrate, which is extracted through the extract, and the extra...

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PUM

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Abstract

The invention relates to a method for preparing mono sialic acid hexose ganglioside sodium agent, comprising removing integument and blood vessel from the fresh or frozen pig brain or cattle brain, pushing for dewatering with cold acetone for several times, filtering with organic membrane, pouring out the acetone, and treating into acetone powder, packing, and conserving; extracting clarified solution from acetone powder, filtering with ultra-filter membrane and getting ultra-filtrate, extracting with tobacco extract, sodium filtering with sodium filter membrane to remove small molecule, abandoning the filtrate, getting clean condensed liquid of the mono sialic acid hexose ganglioside sodium agent; sterilizing and getting the refine product. The invention changes the polarity of the extracting organic solvent, which makes the ganglioside be released almost fully into the solution. The invention employs the ionic sodium filter membrane to separate and converse the ganglioside depending on the its number difference in sialic acid and carboxyl groups, and employs modern membrane separation technique to replace filling material and column suction technique, which saves large quantity of solvent and time, and increases productivity.

Description

technical field [0001] The invention relates to a preparation method of medicine, in particular to a preparation method of separating and extracting glycolipid substances with monosialotetrahexosyl ganglioside sodium (GM1) as the main component from animal tissues. Background technique [0002] Gangliosides (GLS) are a class of acidic glycolipids containing sialic acid (N-acetylneuraminic acid), which are important components of mammalian cell membranes. The membrane glycolipids are composed of a hydrophobic ceramide moiety and a hydrophilic sialooligosaccharide group. Ganglioside molecules are both water-soluble and fat-soluble, and are amphipathic molecules; gangliosides are divided into different types according to the position of sialic acid residues and the length of the oligosaccharide core region, among which GM1 is a monosialotetrahexosyl ganglioside. It has a variety of important biological functions and has become a hot topic in life science research. Sodium mono...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7032A61K9/19A61P25/00
Inventor 严家定
Owner 严家定
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