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Extraction method of radix glehniae acidic polysaccharide, extract thereof and application thereof in immunoregulation

A technology of acidic polysaccharide and extraction method, which is applied in the direction of medical preparations containing active ingredients, drug combinations, pharmaceutical formulas, etc., can solve the problem of not being able to improve the immune activity of macrophages, and achieve high purity, high immune activity, and strong immunity active effect

Inactive Publication Date: 2020-06-23
THE SECOND AFFILIATED HOSPITAL OF CHONGQING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its disadvantages are: 1. The polysaccharides extracted from the reference file are neutral polysaccharides, which cannot improve the immune activity of macrophages compared with the acidic polysaccharides from the reference file; 2. The polysaccharides extracted from the reference file are only effective in liver cancer. Research conducted, no relevant experiments involving lung cancer

Method used

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  • Extraction method of radix glehniae acidic polysaccharide, extract thereof and application thereof in immunoregulation
  • Extraction method of radix glehniae acidic polysaccharide, extract thereof and application thereof in immunoregulation
  • Extraction method of radix glehniae acidic polysaccharide, extract thereof and application thereof in immunoregulation

Examples

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Effect test

Embodiment 1

[0045] Example 1: Extraction of acidic polysaccharide GLP-2a from Adenophora japonicus

[0046] A kind of extraction of the acidic polysaccharide of Adenophora japonicus, concrete steps are as follows:

[0047] 1) Using Adenophora as raw material, crudely extract crude polysaccharides from Adenophora:

[0048] 1-1) Hot water extraction step: Grind the raw material Adenophora in a pulverizer to obtain Adenophora powder; add Adenophora powder in a reaction cup for hot water extraction, then add distilled water I, and place in a water bath at 80°C Stir for 2 hours, collect the extract Ⅰ by filtration; add distilled water Ⅱ to the residue, stir in a water bath at 80°C for 2 hours, and collect the extract Ⅱ by filtration; combine the extract Ⅰ and extract Ⅱ, centrifuge to remove the precipitate, and place in a rotary evaporator Concentrate under reduced pressure to obtain a water-soluble crude extract of Adenophora radix; the weight-number ratio of Adenophora radix powder, distill...

Embodiment 2

[0072] Example 2: Experiments on Immunomodulation and Anti-tumor Effects of Adenophora Adenophora Acidic Polysaccharides in Lung Cancer

[0073]The acidic polysaccharide of Adenophora japonicus used in the experiment was obtained by the above method, with a molecular weight of 337.38kDa; the monosaccharide composition and molar ratio of the acidic polysaccharide of Adenophora japonicus are: mannose: ribose: rhamnose: glucuronic acid: galacturonic acid: Glucose: galactose: arabinose = 0.12:0.74:0.45:1.11:0.88:91.74:2.81:2.2.

[0074] Methods: Detecting the effects of GLP-1a and GLP-2a on the release of NO from macrophages, detecting the effects of GLP-1a and GLP-2a on the production of TNF-α by macrophages, and detecting the effects of GLP-2a on tumor weight in mice , Detect the effect of GLP-2a on the tumor inhibition rate of mice, detect the effect of GLP-2a on the spleen index of mice, and detect the effect of GLP-2a on the thymus index of mice.

[0075] Instruments and mat...

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Abstract

The invention relates to a radix glehniae acidic polysaccharide extraction method, which specifically comprises: 1) adopting radix glehniae as a raw material, and carrying out a hot water extraction step, an ethanol precipitation step, a protein removal step, a dialysis concentration freeze-drying step to prepare radix glehniae crude polysaccharide; (2) extracting radix glehniae polysaccharide from the radix glehniae crude polysaccharide by adopting a DEAE (Diethylaminoethyl) SepharoseTM Fast Flow column chromatography technology; and (3) extracting the radix glehniae acidic polysaccharide from the radix glehniae polysaccharide by adopting a SephacrylTMS-300 High Response column chromatography technology, and indicating that the radix glehniae acidic polysaccharide has a lung cancer immunoregulation anti-tumor effect in a lung cancer immunoregulation anti-tumor experiment.

Description

technical field [0001] The invention relates to the technical field of plant extraction, in particular to a method for extracting acidic polysaccharides from Adenophora japonicus and its extract and application. Background technique [0002] Glehnia littoralis (Glehnia littoralis), also known as Laiyang ginseng, sea ginseng, and silver ginseng, is the dried root of the Umbelliferae plant coral dish. The main active ingredients of Adenophora chinensis are polysaccharides, volatile oils, coumarins and glycosides, among which the content of polysaccharides is the highest. Glehnia littoralis polysaccharides (GLP) not only enhances the immune function of the body, but also has anti-tumor activity, can inhibit the proliferation and migration of lung cancer cells, and can be used as a source of new natural anti-cancer drugs. Studies have shown that many immune-like polysaccharides can play an immunomodulatory role by activating immune cells, especially antigen-presenting cells. T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P35/00A61P37/02
CPCA61K31/715A61P35/00A61P37/02C08B37/0003C08B37/0087
Inventor 鲍依稀张旭裕刘子靖钟诚蒲有为
Owner THE SECOND AFFILIATED HOSPITAL OF CHONGQING MEDICAL UNIV
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