Five ent-atisane type diterpenoid compounds derived from euphorbia antiquorum and preparation method and application of ent-atisane type diterpenoid compounds
A compound, the technology of Huoyele, which is applied in the field of diterpenoids and its preparation, can solve the problems of surrounding tissue and cell damage
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Embodiment 1
[0045] (1) 11.5 kg of Huoyele stems were extracted with methanol three times (amount of 3 × 60 L), and the extract was recovered under reduced pressure to obtain a crude extract;
[0046] (2) Add water to the methanol extract obtained in step (1) to make a suspension, and extract with ethyl acetate and petroleum ether respectively to obtain ethyl acetate and petroleum ether extracts;
[0047] (3) Step (2) The petroleum ether extract was separated by silica gel column chromatography, followed by petroleum ether: acetone 100:0, 100:2, 100:4, 100:6, 100:8, 100:11, 100:16, 100:23, 100:30 elution;
[0048] (3) The petroleum ether:acetone 100:2~100:23 fraction obtained in the above step (2) is separated by medium pressure liquid chromatography (MPLC), using methanol / water 6:4~9:1 as the mobile phase gradient Elution;
[0049] (4) The methanol / water (6:4) and (8:2) fractions obtained in the above step (3) were separated by HPLC-RI and eluted with methanol / water 60:40-90:10 as the m...
Embodiment 2
[0067] (1) 8.0 kg of Huoyele whole herb was extracted 3 times with ethanol (amount of 3×48 L), and the extract was recovered under reduced pressure to obtain a crude extract;
[0068] (2) The ethanol extract obtained in step (1) is added with water to make a suspension, and extracted with ethyl acetate to obtain the ethyl acetate extract;
[0069] (3) Step (2) is separated by silica gel column chromatography, and eluted with petroleum ether: acetone 100:4, 100:6, 100:8, 100:11, 100:16, 100:23, 100:30 in sequence;
[0070] (3) Petroleum ether obtained in the above step (2): ethyl acetate 100:2~100:23 fractions are separated by medium pressure liquid chromatography (MPLC), using methanol / water 7:3~9:1 as the flow Phase gradient elution;
[0071] (4) The methanol / water (8:2) fraction obtained in the above step (3) was separated by HPLC-RI, and the new compound 1 was obtained by eluting with methanol / water 70:30~90:10 as mobile phase (yield 0.001 %), 2 (0.002% yield), 3 (0.003% ...
Embodiment 3
[0074] (1) 8.0 kg of Huoyele whole herb was extracted 3 times with acetone (amount of 3 × 48 L), and the extract was recovered under reduced pressure to obtain a crude extract;
[0075] (2) Add water to the acetone extract obtained in step (1) to make a suspension, and extract with dichloromethane to obtain a dichloromethane extract;
[0076] (3) Step (2) is separated by silica gel column chromatography, and eluted with petroleum ether: acetone 100:4, 100:6, 100:8, 100:11, 100:16, 100:23, 100:30 in sequence;
[0077] (3) The petroleum ether:acetone 100:2~100:23 fraction obtained in the above step (2) is separated by medium pressure liquid chromatography (MPLC), using methanol / water 7:3~9:1 as the mobile phase gradient Elution;
[0078] (4) The methanol / water (8:2) fraction obtained in the above step (3) was separated by HPLC-RI and eluted with acetonitrile / water 70:30-90:10 as the mobile phase to obtain the new compound 1 (yield 0.001 %), 2 (0.001% yield), 3 (0.003% yield), ...
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