A copper ion-labeled colloidal gold test strip for detecting endotoxin and its preparation and detection method

Abstract

The invention relates to a copper ion-labeled colloidal gold test strip for detecting endotoxin and a preparation and detection method thereof. A copper ion-labeled colloidal gold test strip for detecting endotoxin, the test strip includes a bottom plate and a sample pad, a connection pad, a nitrocellulose membrane and an absorption pad that are sequentially overlapped and fixed on the bottom plate; Colloidal gold probes labeled with copper ions are fixed on the connecting pad; detection lines and quality control lines are arranged on the nitrocellulose membrane; aptamer capture probes are fixed on the detection lines; Streptavidin is immobilized; the invention also discloses a method for detecting endotoxin by using the above-mentioned copper ion-labeled colloidal gold test strip. The present invention uses copper ion-mediated gold nanoparticle aggregates and high-affinity aptamers as detection probes and capture probes. Compared with traditional colloidal gold test strips, it has strong specificity, low detection limit and high sensitivity. Good color rendering.

Description

technical field [0001] The invention relates to a copper ion-labeled colloidal gold test strip for detecting endotoxin and a preparation and detection method thereof, belonging to the technical field of biochemical detection. Background technique [0002] Endotoxin is one of the components of the outer membrane of Gram-negative bacteria. A small amount of endotoxin can cause elevated body temperature and a series of harmful symptoms, such as diarrhea, asthma, intravascular coagulation, and septic shock. Therefore, rapid and sensitive detection of endotoxin is very important for food safety and medical supplies. [0003] Traditional endotoxin detection methods are mostly based on the Limulus test and the rabbit pyrogen test method, but these two methods are mainly through enzyme-catalyzed reactions, so the determination results are easily affected by enzymes, resulting in inaccurate test results and low sensitivity , High detection limit, long detection time. In recent year...

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Problems solved by technology

However, this method still has the disadvantages of cumbersome operation and long detection time.

[0005] Chinese patent document CN105116142A discloses a new type of bacterial endotoxin detection test paper and detection method. The Phos-tag combines the phosphate group of endotoxin, and the combination of polymyxin B and lipoid A of endotoxin can quickly, conveniently and timely detection, but the patent has the problem that the specificity of the detection test paper is not strong

[0006] At present, the test strips based on copper ion-labeled colloidal gold probes and aptamers as capture probes have not been reported.

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