Tissue culture method of Syringa microphylla and method for rapidly obtaining large seedlings of Syringa microphyllum

A small-leaf clove, tissue culture technology, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve problems such as low axillary bud induction rate, and achieve the effects of large reproduction, high survival rate, and fast speed.

Active Publication Date: 2022-03-04
内蒙古和盛生态科技研究院有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Therefore, the technical problem to be solved in the present invention is to overcome the defect in the prior art that the axillary bud induction rate is low when propagating Lilac microphylla by tissue culture, thereby providing a tissue culture method of Syringa microphylla

Method used

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  • Tissue culture method of Syringa microphylla and method for rapidly obtaining large seedlings of Syringa microphyllum

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Step 1: At the end of April, cut off 7-8cm of the top part of the new young shoots of healthy and pest-free plants. Remove all the leaves from the cut stems and rinse them under running water for 30 minutes. Alcohol will cause a certain degree of damage to the explants. The selected stems are too tender, skip alcohol sterilization, directly soak in sodium hypochlorite solution with 1% available chlorine for 20 minutes, rinse 3 times with sterile water, and dry the stems. Cut to 1-1.5cm and ensure that each stem segment has terminal buds or 1-2 axillary buds, and the explant survival rate is 80%;

[0060] Step 2: Start the cultivation, select a stem segment with a terminal bud or an axillary bud, the length is about 1-1.5cm. When inoculating, the buds should just touch the medium, so that the buds can effectively absorb nutrients and make the seedlings strong in the later subculture process. , with many differentiations. Using MS as the basal medium, add 30g / L sucrose,...

Embodiment 2

[0067] Step 1: Cut off the top part of 7-8cm from the top part of the new young shoots of healthy and pest-free plants in early May. Remove all the leaves from the cut stems and rinse them under running water for 30 minutes. Alcohol will cause a certain degree of damage to the explants. The selected stems are too tender, skip alcohol sterilization, directly soak in sodium hypochlorite solution with 1% available chlorine for 20 minutes, rinse 5 times with sterile water, and dry the stems. Cut to 1-1.5cm and ensure that each stem segment has terminal buds or 1-2 axillary buds, and the explant survival rate is 82%;

[0068] Step 2: Initiate culture, select a stem section with terminal buds or two axillary buds, the length is about 1-1.5cm. When inoculating, the buds should be just in contact with the initiation medium, so that the buds can effectively absorb nutrients, so that the seedlings in the later subculture process Strong, well differentiated. Start the medium with MS as...

Embodiment 3

[0075] Step 1: Cut off the top part of 7-8cm from the top part of the new young shoots of healthy and pest-free plants in early May. Remove all the leaves from the cut stems and rinse them under running water for 30 minutes. Alcohol will cause a certain degree of damage to the explants. The selected stems are too tender, skip alcohol sterilization, directly soak in sodium hypochlorite solution with 1% available chlorine for 20 minutes, rinse 5 times with sterile water, and dry the stems. Cut to 1-1.5cm and ensure that each stem segment has terminal buds or 1-2 axillary buds, and the explant survival rate is 82%;

[0076] Step 2: Initiate culture, select a stem section with terminal buds or two axillary buds, the length is about 1-1.5cm. When inoculating, the buds should be just in contact with the initiation medium, so that the buds can effectively absorb nutrients, so that the seedlings in the later subculture process Strong, well differentiated. Start the medium with MS as...

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Abstract

This application relates to the tissue culture method of Lilac microphylla and the method for rapidly obtaining large seedlings of Lilac microphylla. The tissue culture method comprises the following steps: (1) selection and disinfection of explants: cutting the top part of the young shoots of the year, and cutting it into Have the stem section of terminal bud or 1-2 axillary bud as explant, carry out disinfection; Germination of terminal buds or axillary buds; the starting medium is based on MS medium, and 30g / L of sucrose, 6g / L of agar, 1mg / L of hormone BA, and 0.2mg / L of hormone NAA are added at the same time, and the pH is adjusted to 5.8-6.2 (3) subculture; (4) rooting culture; (5) transplanting to obtain tissue culture seedlings. The method makes the explant of the lilac lobata explants have a high survival rate during the initiation culture process, is not easy to produce callus tissue, and has a high induction rate of axillary buds or terminal buds.

Description

technical field [0001] The invention relates to the field of asexual propagation of Lilac microphyllum, in particular to a tissue culture method of Lilac microphyllum and a method for rapidly obtaining large seedlings of Lilac microphyllum. Background technique [0002] Small leaf clove (Syringa microphylla Diels), aliases are also called Four Seasons Clove, Second Degree Plum, Wild Clove and so on. The family and genus is Oleaceae Syringa, which belongs to deciduous shrubs, about 2.5m high, and the young branches are taupe and pilose. Leaves ovate or elliptic-ovate, entire, ciliate. Panicles loose, lateral, lavender-red. The leaves of lilac are smaller than ordinary lilac, the branches are lower, the branches are soft and thin, the tree is beautiful, the flower color is bright, and it blooms twice a year, which solves the current situation of no flowers in summer and autumn, and is an excellent flowering shrub in gardens. It is suitable for planting in gardens, residenti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 李瑞静田菊
Owner 内蒙古和盛生态科技研究院有限公司
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