Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Rheumatoid arthritis diagnostic marker and application thereof

A diagnostic marker, rheumatoid technology, applied in the direction of disease diagnosis, biological testing, biomaterial analysis, etc., to achieve the effect of easy results

Active Publication Date: 2020-08-18
SUN YAT SEN UNIV
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As for the role of p38-binding protein in the diagnosis of rheumatoid arthritis, there are no related research reports

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Rheumatoid arthritis diagnostic marker and application thereof
  • Rheumatoid arthritis diagnostic marker and application thereof
  • Rheumatoid arthritis diagnostic marker and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Detection of p38IP protein level in peripheral blood mononuclear cells of RA patients

[0034] 1. Human peripheral blood isolation Human peripheral blood mononuclear cells

[0035] (1) Peripheral blood samples from healthy people or RA patients were donated by the General Hospital of Guangzhou Military Command, and human peripheral blood (with anticoagulant added) was mixed with equal volumes of Hanks solution;

[0036] (2) After adding 15ml of Ficoll solution into a 50ml centrifuge tube, carefully add the peripheral blood-Hanks mixture to the upper layer of Ficoll solution with a dropper, pay attention to maintain the layered interface, and add to 45ml;

[0037] (3) Centrifugation: centrifuge at 2000rpm for 20min. After centrifugation, the solution is divided into 3 layers, and there is a layer of white misty mononuclear cell group at the boundary between the upper layer and the middle layer, which is called the buffy coat layer;

[0038] (4) Carefully inse...

Embodiment 2

[0052] Example 2 Detection of p38IP mRNA level in peripheral blood mononuclear cells of RA patients

[0053] 1. Extraction of total cellular RNA

[0054] Follow the instructions of MagZol reagent from Magen Company:

[0055] (1) Take at most 1×10 7 Amount of PBMC cells (from healthy people or RA patients), centrifuged at 200g for 5min to obtain cell pellet;

[0056] (2) Add 1mL MagZol to resuspend the cell pellet and pipette several times to fully lyse the tissue or cells, and incubate at room temperature for 3-5min;

[0057] (3) Add 200 μL of chloroform, shake vigorously for about 15 seconds, and incubate at room temperature for 3 minutes;

[0058] (4) Centrifuge at 12000g for 15min at 4°C;

[0059] (5) Carefully transfer the upper aqueous phase to a new EP tube, add an equal volume of isopropanol, mix by inversion, and incubate at room temperature for 10 minutes to precipitate RNA;

[0060] (6) Centrifuge at 12000g for 10min at 4°C;

[0061] (7) Discard the supernatant...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention discloses a rheumatoid arthritis diagnosis marker and an application thereof. A marker is p38IP; the rheumatoid arthritis can be diagnosed by detecting the concentration level of p38IP in peripheral blood mononuclear cells. According to the invention, the protein level of p38IP is detected by utilizing immunoblotting or the mRNA level of p38IP is detected by utilizing fluorescent quantitative PCR (Polymerase Chain Reaction); risk evaluation and preliminary diagnosis are carried out on rheumatoid arthritis, the method has the advantages of being easy and convenient to operate, easy to judge and the like, a new thought is provided for research and treatment of rheumatoid arthritis, and the method has a large application prospect.

Description

technical field [0001] The invention relates to the technical field of biological detection, and more specifically, to a diagnostic marker for rheumatoid arthritis and its application. Background technique [0002] Rheumatoid arthritis (RA) is a typical autoimmune disease, and its pathophysiology is characterized by chronic inflammation of the synovium, which can destroy articular cartilage and paraarticular bone. An important feature of rheumatoid arthritis is the infiltration of T cells, B cells, and monocytes in the synovium of multiple joints. The expansion of synovial fibroblast-like and macrophage-like cells leads to synovial hyperplasia, and this dilated synovium is often capable of invading the periarticular bone at the cartilage-bone junction, resulting in bone erosions and cartilage degeneration. If left untreated or unresponsive to treatment, inflammation and joint destruction can lead to loss of physical function, seriously affecting the patient's quality of lif...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883G01N33/68G01N33/53C12N15/11C07K16/18
CPCC12Q1/6883G01N33/6893G01N33/6854G01N33/53C07K16/18C12Q2600/158G01N2800/102
Inventor 李迎秋赵晨思王旭东
Owner SUN YAT SEN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com