Antibody array card for detecting oxaprozin and ibuprofen by using marker guided universal signal

A marker, array card technology, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of unstable quality, low sensitivity, weak detection signal strength, etc., to improve detection sensitivity and quantitative precision, improve Detects signal strength and sensitivity, the effect of increasing signal strength and stability

Pending Publication Date: 2020-08-21
SHAOGUAN COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method has the following disadvantages: 1. To detect different target substances, it is necessary to use corresponding detection antibodies to connect quantum dot microspheres to prepare fluorescent probes, which requires a large workload, low yield, and unstable quality; The reaction to prepare fluorescent probes may affect the structure and immunoaffinity of antibodies; 3. Lack of signal amplification mechanism, the detection signal intensity is weak and the sensitivity is low
However, the immunogenicity of the "detection antigen carrier protein" is unstable, and slight changes in protein conformation will lead to a decrease in the affinity between the "universal antibody" and the "detection antigen", weaken the formation of "sandwich immune complexes", and affect the detection signal. produce

Method used

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  • Antibody array card for detecting oxaprozin and ibuprofen by using marker guided universal signal
  • Antibody array card for detecting oxaprozin and ibuprofen by using marker guided universal signal
  • Antibody array card for detecting oxaprozin and ibuprofen by using marker guided universal signal

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] A label-guided universal signal detection antibody array card for oxaprozine and buprofen, comprising a liner 5, and bifunctional antigen release pads 1, Universal fluorescent probe release pad 2, nitrocellulose membrane 3 (chromatographic membrane) and water-absorbent pad 4; The detection antigen is based on the coupling of "7-amino-4-hydroxy-2-naphthalenesulfonic acid" label 62; the universal fluorescent probe release pad 2 contains anti-"7-amino-4-hydroxy-2 - the universal fluorescent probe of naphthalenesulfonic acid antibody 65, the universal fluorescent probe is quantum dot 61; the nitrocellulose membrane 3 is provided with detection line A, detection line B and quality control line 33, the detection line A, detection line B respectively immobilized anti-oxaprozin antibody 311 and anti-bunofen antibody 321, and the quality control line 33 immobilized anti-"7-amino-4-hydroxy-2-naphthalenesulfonic acid" antibody 65.

[0041] refer to figure 1 In this example, the ...

Embodiment 2

[0050] Simultaneously detect the detection method of oxaprozine and ibuprofen, the steps are as follows:

[0051] Take 0.2g (or 0.2mL) sample, dissolve it in 2.0mL absolute ethanol to fully extract the target sample, let the extract stand for 10 minutes to fully precipitate impurities, take 0.2mL supernatant and add it to 20mL sample buffer, fully Shake well to form a sample solution; drop 3-4 drops of sample solution on the bifunctional antigen release pad with a dropper, and the bifunctional antigen and universal fluorescent probe are released during the movement of the sample solution to the nitrocellulose membrane, and pass through the detection area successively. Line and quality control line, according to the fluorescent signal of detection line A, detection line B and quality control line to determine whether the sample contains oxaprozine and ibuprofen.

[0052] refer to Figure 5. The anti-oxaprozin antibody 311 immobilized on the nitrocellulose membrane detection l...

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Abstract

The invention relates to an antibody array card for detecting oxaprozin and ibuprofen by using a marker guided universal signal. The antibody array card comprises a lining plate, and a difunctional antigen release pad, a universal fluorescent probe release pad, a chromatographic membrane and a water absorption pad which are sequentially adhered to the lining plate and partially overlapped with oneanother, wherein the bifunctional antigen release pad comprises a specific marker coupled on the basis of a detection antigen formed by coupling ovalbumin with oxaprozin and ibuprofen respectively; the universal fluorescent probe release pad comprises a universal fluorescent probe labeled with an anti-specific marker antibody; the chromatographic membrane is provided with a detection line A, a detection line B and a quality control line which are respectively used for fixing an anti-oxaprozin antibody, an anti-ibuprofen antibody and the anti-specific marker antibody. According to the invention, the production of all detection reagents can be solved by using the universal fluorescent probe, the cost is reduced, and the quality control reliability is improved; and the signal intensity and the stability of detection are improved, and the detection sensitivity and the quantitative precision are improved.

Description

technical field [0001] The invention relates to the field of food and drug safety detection, in particular to an antibody array card for marker-guided universal signal detection of oxaprozine and buprofen. Background technique [0002] Both oxaprozine and ibuprofen are non-steroidal anti-inflammatory drugs with anti-inflammatory, analgesic and antipyretic effects. Because these two kinds of analgesic effects are quick and cheap, unscrupulous manufacturers illegally add oxaprozine and ibuprofen to health food (mainly health wine) for rheumatic diseases to enhance the curative effect and seek illegitimate benefits. These two non-steroidal anti-inflammatory drugs are prescription drugs. Improper use may cause damage to the digestive tract, liver and kidneys, and even lead to anaphylactic shock and acute renal failure in severe cases. Long-term consumption of health food containing oxaprozine and ibuprofen without the knowledge of patients is very harmful, and the supervision a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/58G01N33/558
CPCG01N33/9486G01N33/588G01N33/582G01N33/558
Inventor 郭杰标
Owner SHAOGUAN COLLEGE
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