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Preparation method and application of hyaluronic acid-heparin adhered great saphenous vein patch

A technology of hyaluronic acid and heparin, applied in pharmaceutical formulations, coatings, pharmaceutical sciences, etc., to achieve strong antithrombotic effect, small water contact angle, and reduced thickness.

Active Publication Date: 2020-09-29
THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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  • Preparation method and application of hyaluronic acid-heparin adhered great saphenous vein patch
  • Preparation method and application of hyaluronic acid-heparin adhered great saphenous vein patch
  • Preparation method and application of hyaluronic acid-heparin adhered great saphenous vein patch

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Embodiment

[0027] A preparation method of a hyaluronic acid-heparin-adhered great saphenous vein patch, comprising the following steps:

[0028] (1) Pretreatment of tissue materials. The great saphenous vein in the body was taken out and placed in heparin saline at 4°C. After decellularization with 1% SDS, the decellularized large saphenous vein was treated at a temperature of 37°C. The saphenous vein was first adhered to the surface with 2 mg / mL hyaluronic acid at pH = 7 for 15 min, and then adhered to the surface with 2 mg / mL heparin at pH = 7 for 15 min. piece;

[0029] (2) Implanted into the body, the above-mentioned hyaluronic acid-heparin-adhered decellularized great saphenous vein patch was analyzed by electron microscope and water contact angle change, and after the hyaluronic acid-heparin was successfully adhered, the above-mentioned hyaluronic acid-heparin Adhesive decellularized great saphenous vein patch was implanted in vivo.

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Abstract

The invention relates to the technical field of intimal hyperplasia, in particular to a preparation method and application of a hyaluronic acid-heparin adhered acellular great saphenous vein patch. The preparation method of the hyaluronic acid-heparin adhered great saphenous vein patch comprises the following steps of (1) taking out great saphenous veins in vivo, putting the great saphenous veinsin heparin saline at 4 DEG C, and carrying out decellularization treatment by adopting 1% SDS; adopting hyaluronic acid surface adhesion for the decellularized great saphenous vein, and the adopting the heparin surface adhesion to prepare a hyaluronic acid-heparin adhered decellularized great saphenous vein patch; and (2) implanting the hyaluronic acid-heparin adhered acellular great saphenous vein patch in vivo. After the hyaluronic acid-heparin is adhered to the acellular great saphenous vein, the surface of the great saphenous vein becomes smooth; the hyaluronic acid-heparin adhered acellular human great saphenous vein patch has a relatively strong antithrombotic effect in vitro; and in vivo, intimal hyperplasia thickness can be significantly reduced after venous and arterial patch formation.

Description

technical field [0001] The invention relates to the technical field of vascular intimal hyperplasia, in particular to a preparation method and application of a hyaluronic acid-heparin-adhered decellularized great saphenous vein patch. Background technique [0002] Vascular intimal hyperplasia and the resulting stenosis of the official lumen are common physiological features of cardiovascular diseases such as hypertension and restenosis after angioplasty. They are closely related to vascular remodeling and will eventually affect the function of target organs. The pathogenesis of vascular intimal hyperplasia is very complex, and currently it is believed that it may be related to many factors such as endothelial dysfunction and injury, platelet aggregation and thrombus formation, migration and proliferation of vascular smooth muscle cells to the intima, and synthesis of a large amount of extracellular matrix. Vascular intimal hyperplasia has afflicted patients for a long time a...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/34A61L27/50A61L27/54
CPCA61L27/34A61L27/3633A61L27/50A61L27/507A61L27/54A61L2300/232A61L2300/42A61L2300/606A61L2400/18A61L2420/02C08L5/08C08L5/10
Inventor 白华龙李敬安
Owner THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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