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DNA barcoding sequence and method for identifying Lycium barbarum species by using same

A barcode and Lycium barbarum technology, applied in biochemical equipment and methods, microbial measurement/inspection, etc., can solve problems such as lagging in excavation and utilization of excellent Lycium barbarum resources, lack of species richness and quantity in barcode database, and unclear relationship between species

Pending Publication Date: 2020-10-02
WOLFBERRY ENG RES INST NINGXIA ACADEMY OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention aims at the problems in the prior art that the mining and utilization of wolfberry excellent resources are lagging behind, the genetic background of wolfberry germplasm resources is unclear, the relationship between species is unclear, and the species of wolfberry with similar external forms, habits, and tissue structures cannot be distinguished, etc. Due to the relative lack of species richness and quantity in the barcode database of Lycium barbarum, relying on the only domestic Lycium barbarum germplasm resource nursery and the breeding materials obtained from long-term development of Lycium barbarum new varieties, it also provides black fruit Lycium barbarum, yellow fruit wolfberry, round fruit wolfberry, red branch wolfberry and other varieties, Ningxia wolfberry local varieties, Sichuan, northern, Xinjiang, Yunnan, Hebei and other local varieties and hybrid populations, aerospace mutation populations, ploidy populations and other sources of wolfberry A rapid molecular marker identification method for representative germplasm of Lycium barbarum, which can be applied to the identification of Lycium barbarum species

Method used

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  • DNA barcoding sequence and method for identifying Lycium barbarum species by using same
  • DNA barcoding sequence and method for identifying Lycium barbarum species by using same
  • DNA barcoding sequence and method for identifying Lycium barbarum species by using same

Examples

Experimental program
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Effect test

Embodiment 1

[0067] Example 1 Identification of wolfberry samples and construction of psbH-petB barcode database for wolfberry samples

[0068] The present invention will be further described below using examples of implementation.

[0069] 1. Lycium barbarum sample psbH-petB barcode database sample

[0070] In order to construct the psbH-petB barcode database of wolfberry samples, a total of 24 wolfberry samples with similar shapes from different regions were collected. See Table 1 for details:

[0071] Table 1 Lycium barbarum samples (psbH-petB barcode database of Lycium barbarum samples)

[0072]

[0073] (Note: Variety numbers 11, 22-23 are derived from public literature 1: "Wan Ru, Wang Yajun, An Wei, et al. Identification of 21 Lycium barbarum plants based on the psbA-trnH sequence barcode [J]. Jiangsu Agricultural Science, 2019, 47(01):64-67.”; the variety number 13 is derived from the public document 2: “Shi Zhigang. Genetic diversity of 18 Lycium barbarum resources in Ningxia...

experiment example 1

[0121] Experimental Example 1 Using Barcode Database to Identify Lycium Varieties

[0122] 1. Sampling

[0123]The selection numbers are Ningqi No. 2, Ningqi No. 5, Ningqi No. 7, Mengqi No. 1, Ningqicai No. 1, AN-YN-02, strain 6, Dahuangguo, Xiaomaye, 332, HZ- 18 copies of 13-01, ZH-13-08, W-12-27, W-11-15, ZH-13-08-02, W-13-30, W-13-26, W-12-26 The wolfberry samples to be tested were compared and identified with the barcodes of the psbH-petB barcode database of some wolfberry samples in Example 1. The above-mentioned 18 wolfberry samples to be tested cannot be distinguished simply by their appearance, structure and color, and cannot be identified by morphological methods. Identification. In this experimental example, DNA barcoding technology was used for identification.

[0124] Table 4 Sample numbers and origin information of wolfberry to be tested

[0125]

[0126] 2. DNA extraction and concentration detection, PCR amplification, PCR product cloning, sequence sequenc...

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Abstract

The invention discloses a DNA barcoding sequence and a method for identifying Lycium barbarum species by using the same, and belongs to the technical field of identification of Lycium barbarum species. According to the method, DNA barcoding of psbH-petB of Lycium barbarum is used to identify Lycium barbarum species and determine the interspecific relationship of Lycium barbarum, meanwhile, a psbH-petB barcoding database is obtained on the basis of the method, through comparison of a psbH-petB sequence of a sample to be identified with a sequence in the psbH-petB barcoding database, the Lyciumbarbarum species can be identified effectively, the interspecific relationship of the Lycium barbarum can be determined, and an effective basis is provided for the Lycium barbarum varieties.

Description

technical field [0001] The invention belongs to the technical field of wolfberry species identification, and in particular relates to a DNA barcode sequence and a method for identifying wolfberry species. Background technique [0002] Ningxia wolfberry (Lycium barbarum L.) is a rare and authentic medicinal material in my country. It has anti-oxidation, anti-tumor, softening blood vessels, lowering fat, lowering blood sugar, spermatogenic, liver protection, eyesight, and enhancing human immunity. Since authentic and non-authentic medicinal materials often have the same base or are closely related, they are highly similar in appearance, habit, tissue structure and chemical composition, and traditional identification methods are difficult to make accurate identification. . [0003] DNA barcoding (DNA barcoding) is a new technology that uses one or a few DNA fragments to identify and identify target species. It has the characteristics of simple operation, high accuracy, and rap...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12Q1/6869
CPCC12Q1/6895C12Q1/6869C12Q2531/113C12Q2535/122C12Q2537/165
Inventor 石志刚万如王秀英张曦燕李云翔杨利斌王孝马婷慧
Owner WOLFBERRY ENG RES INST NINGXIA ACADEMY OF AGRI & FORESTRY SCI
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