Enzyme-linked immunosorbent assay diluent for whole blood and preparation method and use method thereof

Abstract

The invention discloses an enzyme-linked immunosorbent assay diluent for whole blood and a preparation method and a use method thereof. The diluent is prepared from disodium hydrogen phosphate, monopotassium phosphate, a surfactant S9, PEG-6000, calcium chloride, glycerol, an anti-rheumatoid factor antibody and a preservative. The invention provides a sample diluent for diluting whole blood. Wholeblood is treated by the diluent, so that the influence of a whole blood sample on a detection result can be effectively removed, meanwhile, a background can be effectively reduced, false positive canbe removed, the detection sensitivity can be improved, the positive and negative contrast ratio can be increased, and the performance is obviously superior to that of a traditional sample diluent fordiluting serum or plasma. The diluent can be used for directly diluting whole blood, the process of extracting serum or plasma is reduced, the operation is convenient to simplify, certainly, the diluent can also be used for diluting serum, and the effect is good.

Description

technical field [0001] The invention relates to the technical field of biological detection reagents, in particular to an enzyme-linked immune diluent for whole blood and a preparation method and application method thereof. Background technique [0002] Enzyme-linked immunoassay kit (ELISA kit) is the most widely used detection technology in enzyme immunoassay technology. The basic method is to adsorb the known antigen or antibody on the surface of the solid phase carrier (polystyrene micro-reaction plate), add the analyte, and then use the enzyme-labeled antigen and antibody to react on the solid phase surface, and wash the liquid The free components in the phase are washed out. ELISA kits usually include coated plates, sample diluents, washing solutions, enzyme-labeled antibody diluents, stop solutions and other reagents used in the detection process. [0003] Most of the current enzyme-linked immunoassay product testing samples are serum or plasma. It is rare to use who...

AI Technical Summary

Problems solved by technology

[0003]The current enzyme-linked immunoassay product testing samples are mostly serum or plasma, and it is rare to use whole blood samples directly, and serum can be better tested after blood coagulation Separation, blood coagulation is generally placed at 4°C for several hours or even longer, and the blood serum can only be obtained after centrifugation after blood coagulation, so the process is cumbersome and time-consuming

However, some components of whole blood samples will affect the test results, increase the background and lead to false positives, and reduce the sensitivity of the test.

Images