Method for maintaining antigen activity in antigen-fluorescent microsphere conjugate and application in novel coronavirus antibody chromatography detection reagent

Abstract

The invention discloses a method for maintaining antigen activity in an antigen-fluorescent microsphere conjugate and application in a novel coronavirus antibody chromatography detection reagent, andsolves the problem that in the existing antigen coupling, after the antigen is coupled to the surface of the microsphere, the protein conformation cannot extend possibly because of steric hindrance and thus the antigen activity is reduced, a part with immunocompetence in an antigen structure is extremely likely to participate in a coupling chemical reaction to cause reduction of the antigen activity and thus the sensitivity of the antibody detection reagent is low. The method comprises the following steps: coupling fluorescent microspheres with an antigen to obtain an antigen-fluorescent microsphere conjugate, and then coupling the antigen again to obtain the antigen-antigen-fluorescent microsphere conjugate. The method has the advantages of high antigen activity of novel coronavirus, sensitive detection, high accuracy and the like.

Description

technical field [0001] The invention relates to the technical field of immunochromatography, in particular to a method for maintaining antigen activity in an antigen-fluorescent microsphere conjugate and its application in a novel coronavirus antibody chromatography detection reagent. Background technique [0002] At present, the detection methods for the new coronavirus are mainly divided into two categories, one is the direct detection of the virus, such as the "gold standard" nucleic acid detection of etiology, which is the detection of certain specific nucleic acid sequences in the virus gene; the other is the indirect detection of the virus. Detection, such as the detection of new coronavirus-specific serum antibodies, when the virus invades the human body, the human immune system will produce a large number of immunoglobulins, the most common of which are IgG and IgM, which are key components of the body's immune response to the virus, so through Serum testing for susp...

AI Technical Summary

Problems solved by technology

[0008] The technical problem to be solved by the present invention is: in the coupling of the existing antigen, after the antigen is coupled to the surface of the microsphere, it may also be due to steric hindrance, so that the protein conformation cannot be stretched and the activity of the antigen is reduced. The active part is very likely to participate in the coupling chemical reaction, resulting in a decrease in the activity of the antigen, resulting in lower sensitivity of the antibody detection reagent

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