Composition for inducing plant disease damage resistance or preventing plant disease damage
A technology for inducing plants and compositions, applied in the direction of chemicals for biological control, plant growth regulators, botany equipment and methods, etc., capable of solving problems such as high harmfulness
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Embodiment 1
[0184] [Example 1] Evaluation of the effect of inducing disease resistance in cucumbers using copper sulfate and amino acids in combination (1)
[0185] In this embodiment, copper sulfate and various amino acids are applied to the first true leaf of cucumber in combination, and the activities of chitinase (Chitinase) and peroxidase (Peroxidase) as disease resistance-related enzymes are used as Indexes to evaluate the effect of disease resistance induction.
[0186] (1) Cultivation of plants and distribution of treatment liquid
[0187] Cucumbers (variety "Siye") were sown into horticultural culture soil (Kumiai Nippi Horticultural Cultivation Soil No. 1; JA) and vermiculite at a volume ratio of 3:1, and cultivated for 2 weeks. The temperature in the culture room was set at 23-25°C, the daytime light was set at 14 hours, and the light intensity was set at about 300 μmol m -2 the s -1 . As the treatment solution, prepare: an aqueous solution containing a combination of 0.32 ...
Embodiment 2
[0196] [Example 2] Evaluation of the effect of inducing disease resistance in cucumbers using copper sulfate and amino acids in combination (2)
[0197] For 7 kinds of amino acids (Thr, Pro, Ala, Val, Ser, Trp, Tyr) and known According to the steps described in Example 1, Lys, which has a bactericidal effect brought about in combination with copper sulfate, was evaluated again for the disease resistance inducing effect brought about in combination with copper sulfate.
[0198] show the result in Figure 9-10 (n=3). In particular, when Thr, Pro, Ala, or Ser was used in combination with copper sulfate, a higher effect of inducing disease resistance was observed than when Lys was used in combination with copper sulfate. Among them, when Ser or Thr was used in combination with copper sulfate, a significantly higher effect of inducing disease resistance was observed.
Embodiment 3
[0199] [Example 3] Evaluation of the influence of the amino acid concentration on the induction of disease resistance by cucumber when used in combination with copper sulfate
[0200] The effect of combined use with copper sulfate on the concentration of each amino acid in the induction of disease resistance by cucumber was evaluated. That is, for the eight kinds of amino acids (Thr, Pro, Ala, Val, Ser, Trp, Tyr, Lys) evaluated in Example 2, the amino acid concentration was set to 0.17mM, 0.36mM, 0.50mM, 0.67mM, 0.84mM or 1.01 mM, according to the steps described in Example 1, the effect of inducing disease resistance brought about by the combined use of copper sulfate was evaluated.
[0201] show the result in Figure 11-26 (n=3). It was confirmed that the effect of inducing disease resistance by the combined use of all amino acids and copper sulfate was concentration-dependent. In addition, it was also shown that the saturation concentration and the maximum effect differ ...
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