Glucose wide-range high-precision automatic detection method and system

Abstract

The invention discloses a wide-range and high-precision automatic detection method for glucose. Based on the fact that the self-made Prussian blue bio-enzyme electrode has a wide range of linearization characteristics for glucose concentration detection, the automatic adjustment of the injection volume according to the self-identified detection area is proposed to achieve a wide range of high-precision detection under an unknown glucose concentration range. The invention adopts the Prussian blue biological enzyme electrode to react with the microbial fermentation liquid in a special detection pool to generate a microampere-level current. By designing a microampere current detection circuit and filtering processing, a response signal and a glucose concentration response equation are established to calculate the measured value of the glucose concentration. , the system independently identifies the detection concentration interval according to the measured value, adjusts the set value V of the injection volume according to the deviation, and automatically injects the sample V through the high-precision intelligent sampling device, so that the concentration of the analyte in the detection pool is at the optimum value of the sensing electrode Response range, to achieve a wide range of glucose, high-precision automatic detection.

Description

technical field [0001] The invention relates to the field of microbial fermentation industry. Aiming at the problem of online detection of the concentration of key components such as substrates, intermediate metabolites, and products in the fermentation process, a wide-range and high-precision automatic detection method for glucose is provided to realize a wide range of glucose at unknown concentrations. The range and precise detection belong to the interdisciplinary technical field of biopharmaceutical, communication and software. Background technique [0002] The "extensive" production mode based on batch fermentation is generally adopted in the field of microbial fermentation industry. During the production process, the concentration changes of substrates or products cannot be tracked in time. The understanding of the fermentation process is often in a "black box" state, and production operations are completely dependent on Due to the lack of operating experience, the pro...

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Problems solved by technology

[0002] The "extensive" production mode based on batch fermentation is generally adopted in the field of microbial fermentation industry. During the production process, the concentration changes of substrates or products cannot be tracked in time. The understanding of the fermentation process is often in a "black box" state, and production operations are completely dependent on Due to the operation experience, the production efficiency is low and the environmental pollution is serious. Therefore, my country urgently needs to develop an online analysis instrument for the concentration of fermentation substrates and products.

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