Glucose wide-range high-precision automatic detection method and system

Abstract

The invention discloses a glucose wide-range high-precision automatic detection method. On the basis that a self-made prussian blue bio-enzyme electrode has a wide-range linearization characteristic on glucose concentration detection, the sample introduction amount is automatically adjusted according to an autonomous recognition detection area, and wide-range high-precision detection under the condition that the glucose concentration range is unknown is achieved. According to the invention, the Prussian blue bio-enzyme electrode is adopted to react with a microbial fermentation broth in a special detection pool so as to generate microampere-level current; a microampere current detection circuit and filtering processing are designed, a response signal and glucose concentration response equation are established, a measurement value of glucose concentration is calculated, a detection concentration interval is automatically identified according to a measurement value system, a sample introduction amount set value V is adjusted according to deviation, and a sample V is automatically introduced through a high-precision intelligent sample introduction device, so that the concentration ofa to-be-detected object in a detection cell is in an optimal response range of a sensing electrode, and wide-range and high-precision automatic detection of glucose is realized.

Description

technical field [0001] The invention relates to the field of microbial fermentation industry. Aiming at the problem of online detection of the concentration of key components such as substrates, intermediate metabolites, and products in the fermentation process, a wide-range and high-precision automatic detection method for glucose is provided to realize a wide range of glucose at unknown concentrations. The range and precise detection belong to the interdisciplinary technical field of biopharmaceutical, communication and software. Background technique [0002] The "extensive" production mode based on batch fermentation is generally adopted in the field of microbial fermentation industry. During the production process, the concentration changes of substrates or products cannot be tracked in time. The understanding of the fermentation process is often in a "black box" state, and production operations are completely dependent on Due to the lack of operating experience, the pro...

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Problems solved by technology

[0002] The "extensive" production mode based on batch fermentation is generally adopted in the field of microbial fermentation industry. During the production process, the concentration changes of substrates or products cannot be tracked in time. The understanding of the fermentation process is often in a "black box" state, and production operations are completely dependent on Due to the operation experience, the production efficiency is low and the environmental pollution is serious. Therefore, my country urgently needs to develop an online analysis instrument for the concentration of fermentation substrates and products.

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