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Molecular marker of ago3 gene related to chicken body weight and abdominal fat weight and its application

A molecular marker and abdominal fat weight technology, applied in the field of molecular biotechnology and molecular markers, can solve the problem of less reporting of Argonaute protein, and achieve the effect of reducing feeding cost, improving profit rate and weight gain.

Active Publication Date: 2021-07-27
SOUTH CHINA AGRI UNIV
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Problems solved by technology

[0005] Little information has been reported on the role of Argonaute proteins in birds, even less in poultry, and especially less in chickens

Method used

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  • Molecular marker of ago3 gene related to chicken body weight and abdominal fat weight and its application
  • Molecular marker of ago3 gene related to chicken body weight and abdominal fat weight and its application
  • Molecular marker of ago3 gene related to chicken body weight and abdominal fat weight and its application

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Embodiment Construction

[0026] The invention will be described in further detail below.

[0027] 1 Experimental materials

[0028] Select F2 full-sib families with deep phenotypic records (Xinghua Chicken × Recessive White Rock Chicken), Xinghua Chicken is a slow-growing native breed, recessive White Rock chicken is an exotic fast-growing broiler breed, Lei et al., 2005Detailed The process of forming the F2 family is introduced.

[0029] 2 Cloning and identification of SNP sites in the 5'-flanking region of chicken AGO3 gene

[0030] 2.1 Cloning of the SNP site in the 5'-flanking region of the chicken AGO3 gene

[0031] The DNA samples of 10 F2 resource populations (Xinghua Chicken × Recessive White Rock Broiler Chicken) were randomly selected from the DNA samples as pool pool templates, and the SNPs in the 5' flanking region of AGO3 were identified by PCR and sequencing. For SNP genotyping, all DNA samples were subjected to PCR and sequencing, and then the resulting sequences were analyzed with t...

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Abstract

The invention discloses a molecular marker of the AGO3 gene related to chicken body weight and abdominal fat weight and its application. The molecular marker is located in the 5′-flanking region of the AGO3 gene on chromosome 23 of the chicken genome, including the following sites: the A>T mutation at 4330286bp on chromosome 23 of gallus gallus version 6 of the chicken genome, and the mutation at 4330299bp on chromosome 23 C>T mutation; when applying, select one or two of these sites for breeding of chicken body weight and abdominal fat weight. By optimizing the dominant genotypes of these SNP sites, the body weight and abdominal fat weight of the chicken can be greatly improved. Under the same feeding conditions, broilers of the same age have heavier body weight, faster daily weight gain, and higher abdominal fat content. , The feed conversion rate is higher, which can not only reduce the feeding cost, but also increase the meat weight, increase the abdominal fat rate, and finally increase the profit margin.

Description

technical field [0001] The invention relates to the technical fields of molecular biotechnology and molecular markers, in particular to an AGO3 gene molecular marker related to chicken body weight and abdominal fat weight and its application. Background technique [0002] The Argonaute protein was identified in plants for the first time. It has two main functional domains, namely the PAZ domain (PiwiArgonauteZwille) and the PIWI domain. The structure of the overall Argonaute protein also includes the MID domain, N-terminal domain and C-terminal domain. The Argonaute protein family of eukaryotes can be divided into AGO protein and PIWI protein, represented by Arabidopsis thaliana AGO1 and Drosophila melanogaster Piwi respectively. They are highly conserved among species, and many organisms encode multiple members of the AGO protein family. [0003] Argonaute proteins perform a variety of biological functions across species. Argonaute proteins are important components of th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156C12Q2600/172
Inventor 聂庆华陈小兰陈扬风黎镇晖徐海平郑茗
Owner SOUTH CHINA AGRI UNIV
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