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AGO3 gene molecular marker related to chicken weight and abdominal fat weight and application of AGO3 gene molecular marker

A technology of molecular markers and abdominal fat weight, which is applied in the field of molecular biotechnology and molecular markers, can solve the problems such as the lack of reports of Argonaute protein, and achieve the effects of reducing feeding costs, increasing meat weight, and high abdominal fat content

Active Publication Date: 2020-12-18
SOUTH CHINA AGRI UNIV
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AI Technical Summary

Problems solved by technology

[0005] Little information has been reported on the role of Argonaute proteins in birds, even less in poultry, and especially less in chickens

Method used

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  • AGO3 gene molecular marker related to chicken weight and abdominal fat weight and application of AGO3 gene molecular marker
  • AGO3 gene molecular marker related to chicken weight and abdominal fat weight and application of AGO3 gene molecular marker
  • AGO3 gene molecular marker related to chicken weight and abdominal fat weight and application of AGO3 gene molecular marker

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Embodiment Construction

[0026] The invention will be described in further detail below.

[0027] 1 Experimental materials

[0028] Select F2 full-sib families with deep phenotypic records (Xinghua Chicken × Recessive White Rock Chicken), Xinghua Chicken is a slow-growing native breed, recessive White Rock chicken is an exotic fast-growing broiler breed, Lei et al., 2005Detailed The process of forming the F2 family is introduced.

[0029] 2 Cloning and identification of SNP sites in the 5′-flanking region of chicken AG03 gene

[0030] 2.1 Cloning of the SNP site in the 5'-flanking region of the chicken AG03 gene

[0031] The DNA samples of 10 F2 resource populations (Xinghua Chicken × Recessive White Rock Broiler Chicken) were randomly selected from the DNA samples as pool pool templates, and the SNPs in the 5′ flanking region of AG03 were identified by PCR and sequencing. For SNP genotyping, all DNA samples were subjected to PCR and sequencing, and then the resulting sequences were analyzed with t...

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Abstract

The invention discloses an AGO3 gene molecular marker related to chicken weight and abdominal fat weight and application. The molecular marker is located in an AGO3 gene 5'-flanking region on a No. 23chromosome of a chicken genome, and comprises the following sites: A > T mutation at the 4330286bp position on the No.23 chromosome of the chicken genome gallus 6 version, and C > T mutation at the 4330299bp position on the No.23 chromosome; and during application, one site or two sites are selected to breed chicken weight and abdominal fat weight. By optimizing the dominant genotypes of the SNPloci, the weight and abdominal fat weight of chickens can be greatly increased, under the same feeding condition and in broilers of the same day age, the weight is heavier, the daily gain is faster, the abdominal fat content is higher and heavier, and the feed conversion rate is higher, so that the feeding cost can be reduced, the meat weight can be increased, the abdominal fat rate can be increased, and finally the profit rate is increased.

Description

technical field [0001] The invention relates to the technical fields of molecular biotechnology and molecular markers, in particular to an AGO3 gene molecular marker related to chicken body weight and abdominal fat weight and its application. Background technique [0002] Argonaute protein was identified in plants for the first time. It has two main functional domains, namely PAZ domain (PiwiArgonauteZwille) and PIWI domain. The structure of the overall Argonaute protein also includes MID domain, N-terminal domain and C-terminal domain. The Argonaute protein family in eukaryotes can be divided into AGO protein and PIWI protein, represented by Arabidopsis thaliana AGO1 and Drosophila melanogaster Piwi respectively. They are highly conserved among species, and many organisms encode multiple members of the AGO protein family. [0003] Argonaute proteins perform a variety of biological functions across species. Argonaute proteins are important components of the RNA-induced si...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156C12Q2600/172
Inventor 聂庆华陈小兰陈扬风黎镇晖徐海平郑茗
Owner SOUTH CHINA AGRI UNIV
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