Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for vitrified freezing and thawing of canine ova, and ova frozen and thawed according to same

A vitrification and vitrification technology, applied in the field of vitrification and thawing of canine eggs, freezing and thawing eggs, can solve the problems of less than 5% production efficiency, results and technical shortages, etc., and achieve high nuclear transfer and fertilization. , the effect of reducing toxicity, high nuclear transfer culture effect

Pending Publication Date: 2020-12-22
艾牧克生物技术有限责任公司 +1
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the production efficiency of somatic cell cloned animals is known to be less than 5% (Heyman et al., 2002; Yang et al. 2008)
Therefore, when the experimental dogs in estrus at the same time cannot be ensured, the technique of securing a sufficient number of eggs by vitrification is required, but at present, there is a shortage of such experimental results and techniques

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for vitrified freezing and thawing of canine ova, and ova frozen and thawed according to same
  • Method for vitrified freezing and thawing of canine ova, and ova frozen and thawed according to same
  • Method for vitrified freezing and thawing of canine ova, and ova frozen and thawed according to same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0051] The present invention relates to a method for vitrifying and thawing ovum of a canine animal, performing the process described below: a process of collecting mature ovum of a canine animal (1 process), performing a process of vitrifying the above-mentioned collected ovum (2 process) ; Including: the process of preparing mixed solution, equilibrium solution, and vitrification solution (2 process-1); the process of removing the cumulus cells of the recovered ovum (denuding) (2 process-2); The process of treating the mature ovum without cumulus cells with the mixed solution (2 process-3); adding the balance solution to the mixed solution containing the ovum and treating it, and then adding the balance solution treatment process (2 process-4) ; The process of treating the treated ovum with the balance solution again (2 process-5); the process of moving the treated ovum to the vitrification solution and processing (2 process-6) and placing the ovum in egg freezing The proces...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to a method for the vitrified freezing and thawing of canine ova for somatic cell replication, and more particularly, to: a method for the vitrified freezing and thawingof canine ova; and ova frozen and thawed according to same. Conventional techniques for producing vitrified canine ova are difficult to synchronize due to differences in estrus cycles. Conversely, a method for the vitrified freezing and thawing of canine ova, and ova frozen and thawed according to same, according to the present invention enable collected ova to be synchronized with experiment schedules through a vitrified freezing technique, even when estruses occur on different dates, and thus exhibit a high nuclear transfer and fertilization effect.

Description

technical field [0001] The present invention relates to a method of vitrifying and thawing animal eggs for somatic cell cloning, in more detail, to a method of vitrifying and thawing canine eggs and the frozen-thawed eggs according to the method. Background technique [0002] Somatic cell cloning refers to transplanting the nucleus of the somatic cell to be cloned after removing the nucleus of the egg, thereby preparing a cloned organism having the same genetic information as the somatic cell. Somatic cell cloning is an epoch-making technology that can produce living organisms through somatic cell nuclear transfer without the fertilization process of egg and sperm. At present, somatic cell cloning technology has been widely used in the production of useful animals, the production of transformed animals for the production of expensive drugs, the cloning of endangered animal species, pet cloning, and regenerative medicine. [0003] Somatic cell cloning requires delicate mecha...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02C12N5/075
CPCA01N1/0221A01N1/021A01N1/0268A01N1/0284C12N15/877A61D19/04C12N5/0609A01N1/0205
Inventor 金敏奎南润晳朴江善池国斌李智慧
Owner 艾牧克生物技术有限责任公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com