Mesenchymal stem cell preparation for treating arthritis and preparation method therefor

A technique for mesenchymal stem cells and arthritis, which is applied in the field of mesenchymal stem cell preparations for the treatment of arthritis and the preparation thereof, can solve the problems of inability to fully utilize the biological properties of mesenchymal stem cells, unfavorable arthritis recovery, single cell type, and the like, To achieve the effect of reducing the phenomenon of adhesion and clumping and cell adhesion to the container wall, reducing cell clumping and embolism, and reducing cell loss

Pending Publication Date: 2021-01-08
山东佰傲干细胞生物技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Aiming at the problems in prior art preparations or methods for treating arthritis such as single cell type, long treatment time, inability to fully exert the biological properties of mesenchymal...

Method used

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  • Mesenchymal stem cell preparation for treating arthritis and preparation method therefor
  • Mesenchymal stem cell preparation for treating arthritis and preparation method therefor
  • Mesenchymal stem cell preparation for treating arthritis and preparation method therefor

Examples

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Embodiment 1

[0045] This embodiment provides a mesenchymal stem cell preparation for treating arthritis, including mesenchymal stem cells, metformin-pretreated mesenchymal stem cells, platelet-rich plasma, and a dispersion medium.

[0046] In some embodiments, the mesenchymal stem cells are bone marrow mesenchymal stem cells, umbilical cord mesenchymal stem cells, fat mesenchymal stem cells, and dental pulp mesenchymal stem cells, and the final cell concentration of the mesenchymal stem cell preparation is 10 6 ~10 8 individual / mL, such as 10 6 pcs / mL, 10 7 pcs / mL, 10 8 individual / mL.

[0047] In some embodiments, the ratio of mesenchymal stem cells to metformin-pretreated mesenchymal stem cells is 1:1-4:1, for example, 1:1, 2:1, 3:1, 4:1.

[0048] In some embodiments, the platelet concentration in the platelet rich plasma is 2-3×10 6 pcs / uL, for example 2×10 6 pcs / uL, 2.5×10 6 pcs / uL, 3×10 6 pcs / uL.

[0049] In some embodiments, the volume ratio of the platelet-rich plasma to the...

Embodiment 2

[0053] This embodiment provides a method for preparing a mesenchymal stem cell preparation for treating arthritis, comprising the following steps:

[0054] 1. Isolation and culture of mesenchymal stem cells

[0055] Use conventional methods to extract 50 mL of bone marrow fluid from the puncture point of the anterior iliac spine with a bone puncture needle, and inject it into a 50 mL sterile centrifuge tube. Dilute it with an equal amount of PBS solution containing 20uL / mL heparin, and add an equal volume of lymphocyte separation solution with a specific gravity of 1.073g / mL along the tube wall. Centrifuge at 2000rmp for 30min, and absorb the mononuclear cell layer. Centrifuge and wash with sterile normal saline twice, centrifuge at 1000rmp for 5min, discard the supernatant, and take the precipitate. The bone marrow mesenchymal stem cells obtained above were suspended in α-MEM medium containing 10% fetal bovine serum, and the cells were counted according to 5×10 6 Each / mL w...

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Abstract

The application provides a mesenchymal stem cell preparation for treating arthritis. The mesenchymal stem cell preparation comprises mesenchymal stem cells, metformin-pretreated mesenchymal stem cells, platelet-enriched blood plasma and a dispersing medium. The metformin-pretreated mesenchymal stem cells can be used for improving osteogenesis differentiation capability of the mesenchymal stem cells and facilitating injury restoration. By using the mesenchymal stem cells and the metformin-pretreated mesenchymal stem cells in a mixed manner, secretion of anti-inflammatory factors of the mesenchymal stem cells can be guaranteed, and the tissue restorability of the mesenchymal stem cells can also be guaranteed. The mesenchymal stem cell preparation provided by the invention is applicable to inflammation relieving and impaired tissue restoration of an osteoarthritis disease and can achieve symptom relieving and function recovery of a sufferer.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a mesenchymal stem cell preparation for treating arthritis and a preparation method thereof. Background technique [0002] Arthritis generally refers to inflammatory diseases that occur in human joints and their surrounding tissues and are caused by inflammation, infection, degeneration, trauma or other factors, and can be divided into dozens of types. There are more than 100 million arthritis patients in my country, and the number is increasing. The clinical manifestations are joint redness, swelling, heat, pain, dysfunction and joint deformity. In severe cases, it will lead to joint disability and affect the quality of life of patients. According to statistics, half of the people over the age of 50 in my country suffer from osteoarthritis, and 90% of women and 80% of men over the age of 65 suffer from osteoarthritis. The prevalence rate in my country is 0.34% ...

Claims

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Application Information

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IPC IPC(8): A61K35/28A61K35/16A61K47/22A61K47/36A61K47/42A61P19/02A61P29/00C12N5/0775
CPCA61K35/28A61K35/16A61K47/42A61K47/36A61K47/22A61P19/02A61P29/00C12N5/0662C12N5/0663C12N5/0667C12N5/0664C12N5/0668C12N2509/00C12N2501/998C12N2501/91C12N2500/38C12N2500/84C12N2501/999C12N2501/135C12N2501/15C12N2501/105A61K2300/00
Inventor 高迎凤程蕊苹蒲锋星
Owner 山东佰傲干细胞生物技术有限公司
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