A compound that increases the amount of predation and egg-laying of small flower stinkbugs
A technology of stink bug and compound, which is applied in the field of insect biology and ecology, can solve problems such as affecting the trapping effect and has not yet been found, and achieve the effects of protecting the ecological environment, increasing biodiversity and reducing the amount of use.
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Embodiment 1
[0029] Compound preparation:
[0030] 1.1 Preparation of 50 μg / ml single component solution of the present invention
[0031] Preparation of 50 μg / ml n-hexanal solution: Add 5 μl of the previously prepared 10 μg / μl n-hexanal standard compound into 995 μl of liquid paraffin, and mix evenly;
[0032] Preparation of 50 μg / ml trans-2-nonenal solution: Add 5 μl of the pre-prepared 10 μg / μl trans-2-nonenal standard compound into 995 μl of liquid paraffin and mix evenly;
[0033] Preparation of 50 μg / ml 2-methylbutyraldehyde solution: add 5 μl of 10 μg / μl 2-methylbutyraldehyde standard compound prepared in advance to 995 μl of liquid paraffin, and mix evenly;
[0034] Preparation of 50 μg / ml 1-octen-3-ol solution: add 5 μl of 10 μg / μl 1-octen-3-ol standard compound prepared in advance to 995 μl of liquid paraffin, and mix evenly;
[0035] Preparation of 50 μg / ml ethanol solution: Add 5 μl of the previously prepared 10 μg / μl ethanol standard compound into 995 μl of liquid paraffin, ...
Embodiment 2
[0046] The Antennary Potential Responses of the Mint Bug to Various Components and Mixtures
[0047] 20 µl of sample solution was evenly dropped on a "V" shaped filter paper strip with a length of 5 cm and a width of 0.5 cm, and was placed in a Pasteur dropper. 1cm away from the tentacles. Adjust the continuous gas flow to 124ml / min, stimulate the gas flow to 20ml / min, and each stimulation time to 0.2s. There was an interval of 40 s between the two stimulations to ensure the complete recovery of the antennal sensory organs. Each component and compound were stimulated 3 times in parallel on one antennae to get the average value, and the test was repeated for at least 5 antennae. The EAG relative reaction value of each component and complex was compared with liquid paraffin. For each sample to be tested, the stimulation sequence is liquid paraffin, sample to be tested, and liquid paraffin. Take the average value of the two comparisons before and after.
[0048] EAG relative...
Embodiment 3
[0053] The whole test was carried out in a rectangular glass releaser, which was placed in an artificial climate chamber at a temperature of 25±1°C, a light time of 16:8 h (L:D), and a relative humidity of (70±5)% RH. The treatment group (the compound of the present invention, formula 1, formula 2, and formula 3) released 4 adult stink bugs respectively, placed 80 thrips on four leaves, and treated the compound of the present invention, formula 1, formula 2 or formula 3 Hangs from the top of the glass release. The blank control group did not release the complex, and other conditions were the same as the treatment group. The treatment groups (compound of the present invention, formula 1, formula 2, and formula 3) and the control group (containing only liquid paraffin) were repeated 3 times, and the number of thrips were recorded after 1 day of starvation before the release of the small flower bugs, and 3 days later.
[0054] The results show that if figure 1 As shown, the pre...
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