Pesticide residue detection method for food detection

Abstract

The invention discloses a pesticide residue detection method for food detection. The method comprises the following steps: (1) preparing a reagent; (2) preparing a sample; (3) detecting a reference solution: adding a buffer solution and a butyrylcholine esterase solution into a reaction bottle through extraction equipment, then adding a color developing agent, adding a substrate, transferring thesolution into a cuvette, taking a supernatant prepared in the step (2), the butyrylcholine esterasesolution and the color developing agent, and transferring the solution into the cuvette through the extraction equipment; and (4) detecting a to-be-detected sample solution. Through the up-and-down displacement action of an extraction part, a first needle tube or a second needle tube can be fixed andseparated by the extraction part, different solutions can be sequentially subjected to pipetting, pipetting of different volumes can also be carried out, and in a sealed environment, a pollution problem is avoided; and therefore, in the detection of the reference solution in the step (3), the extraction equipment can flexibly and quickly operate the solution without errors.

Description

technical field [0001] The invention belongs to the technical field of pesticide residue detection, and in particular relates to a pesticide residue detection method for food detection. Background technique [0002] In our country, pesticide residues in vegetables and fruits are extremely serious. The main reason for the excessive pesticide residues in fruits and vegetables is that some farmers use highly toxic pesticides that are strictly prohibited by the state in the cultivation of fruits and vegetables, resulting in excessive pesticide residues. Eating fruits and vegetables with excessive toxic pesticide residues will cause human and animal poisoning incidents and cause great harm to human bodies. At this stage, there are many rapid detection methods for pesticide residues in my country, which are mainly divided into two categories: biochemical detection methods and chromatographic detection methods. The chromatographic detection method is accurate in determining the s...

AI Technical Summary

Problems solved by technology

The chromatographic detection method is accurate in determining the structure, but the determination time is long. The enzyme inhibition rate method in the biochemical determination method is listed as a national recommended standard method because of its fast speed, rapid response, simple operation, and less investment required. It is aimed at the determination of organophosphorus and carbamate pesticides. The enzyme inhibition method is a rapid detection technology. It mainly measures the content of the product after the enzyme hydrolyzes the substrate, and compares the determination result with the standard product. Detection methods, which can be divided into four types: potentiometric titration method, pressure measurement method, pH difference method (△pH) and colorimetric method, but the current enzyme inhibition rate method has low accuracy in determining the structure

Images