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Biomarker group for sepsis, sepsis judgment method and kit

A biomarker and sepsis technology, applied in the field of biomedicine, can solve the problems of reaching a consensus on the correct threshold of PCT and restricting the clinical application of PCT, and achieve the effect of outstanding accuracy.

Pending Publication Date: 2021-02-23
四川生美思达生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Levels of PCT values ​​between 0.1 and 0.5 ng / mL indicate the need for antimicrobial therapy, but there is no international consensus on the correct threshold for PCT
In addition, the level of PCT is correlated with the severity of the disease in patients with severe pneumonia, and the decrease of PCT level is associated with the improvement of survival rate, which limits the clinical application of PCT

Method used

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  • Biomarker group for sepsis, sepsis judgment method and kit
  • Biomarker group for sepsis, sepsis judgment method and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] This example relates to a panel of biomarkers for sepsis.

[0031] Taking humans as biological sample collectors, biological samples can be whole blood, buffy coat, plasma, serum, peripheral blood mononuclear cells, rod cells, neutrophils, monocytes or T cells, such as Collection is performed by a known method such as whole blood collection. Biomarkers TLR5, CD59, CLU, FGL2, IL7R and CPVL were extracted from biological samples to form a biomarker panel.

[0032] The extraction method of the biomarker group can adopt known methods such as extraction of total RNA from animal tissues.

Embodiment 2

[0034] This example relates to a panel of biomarkers for sepsis.

[0035] The biomarker group may be the amplified RNA fragments of the biomarkers TLR5, CD59, CLU, FGL2, IL7R and CPVL described in Example 1. For example, the amplified RNA fragments shown in Table 1 below.

[0036]

[0037]

Embodiment 3

[0039] This embodiment relates to a method for judging sepsis using a biomarker panel.

[0040] According to the score of measuring the expression level of the biomarker of the subject, it can be judged by the established index system, sepsis infection, suspected sepsis infection and non-infectious inflammation can be distinguished, combined with known clinical data Clinical trials currently underway can speculate that the expected model accuracy will be close to 80%.

[0041] First, measure the expression of biomarkers TLR5, CD59, CLU, FGL2, IL7R, and CPVL in biological samples, such as microarray analysis, polymerase chain reaction, reverse transcriptase polymerase chain reaction, Northern blot, or gene expression series Methods such as analysis can be used to measure biomarkers in biological samples. For different sepsis infection conditions, the expression levels of biomarkers TLR5, CD59, CLU, FGL2, IL7R and CPVL were different, especially when the expression levels of th...

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Abstract

The invention discloses a biomarker group for sepsis, a sepsis judgment method and a kit. The biomarker group is composed of new biomarkers different from an existing RNA markers. When sepsis diagnosis is carried out, through an established index system of the biomarker group, the score of the expression quantity of the biomarker group of a measured subject is judged, so as to identify and stratify the sepsis patient, and provide clues for understanding the main pathophysiological mechanism and the potential targeted therapy approach of a single patient.

Description

technical field [0001] The present invention relates to a biomarker group for sepsis, a sepsis judging method and a kit, and specifically relates to a combination of biomarkers for assisting the diagnosis or treatment of sepsis, and the use of the biomarker group to carry out A method for judging sepsis and a corresponding kit belong to the field of biomedical technology. Background technique [0002] Sepsis is defined by the presence of systemic inflammatory response syndrome (SIRS) with a known or suspected source of infection. However, judging from its clinical manifestations, SIRS does not completely belong to sepsis, but can also be an aseptic inflammation accompanied by non-specific reactions to trauma, surgery, thrombosis and other non-infectious injuries, but it is difficult to treat it clinically. Differentiate systemic inflammation from noninfectious sources, such as tissue trauma. A large amount of data shows that severe sepsis and shock are one of the main caus...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883
CPCC12Q1/6883C12Q2600/158
Inventor 不公告发明人
Owner 四川生美思达生物科技有限公司
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