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Detection kit for NK/T cell lymphoma related genes and library building method

A detection kit and lymphoma technology, applied to the determination/testing of microorganisms, biochemical equipment and methods, etc.

Pending Publication Date: 2021-02-26
RUIJIN HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, research in the field of molecular biology targets related to prognosis is still a blank

Method used

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  • Detection kit for NK/T cell lymphoma related genes and library building method
  • Detection kit for NK/T cell lymphoma related genes and library building method
  • Detection kit for NK/T cell lymphoma related genes and library building method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0084] The composition of the kit of the embodiment of the present invention is shown in Table 1: Diying biological products (article numbers L1001A, LP2004A, C1001A, CP3004A, Y1016A) were used.

[0085] Table 1

[0086]

[0087] The design method of the capture probe is:

[0088] S1. Extract the exon coordinates of the target gene according to the database, and the coordinates of the exon region are shown in Figure 1;

[0089] S2. Intercept 1-120 bp of an exon region as the first probe unit, intercept 60-180 bp of the exon region as the second probe unit, and intercept 120-240 bp of the exon region as the third probe unit For the probe unit, 180-300 bp of the exon region is intercepted as the fourth probe unit, which is successively intercepted to obtain the probe of the exon region;

[0090] S3. Evaluate the nucleotide sequence of the probe, and the evaluated parameters include: Tm value, GC content, Hairpin free energy, sequence complexity and homology within the genom...

Embodiment 2

[0186] 1. Using the kit and method of Example 1 of the present invention to detect 4 samples, the experimental results and data obtained are as follows. The sample information is shown in Table 16:

[0187] Table 16

[0188] Sample No. Concentration (ng / μL) sample type Sample description 1 173.6 dna Clinically diagnosed as NK-TCL 2 8.26 Paraffin-embedded samples Clinically diagnosed as NK-TCL 3 71.0 ascites Clinically diagnosed as NK-TCL 4 78.0 cellular gDNA human T cell leukemia cells

[0189] 2. The experimental results after fragmentation are shown in Table 17:

[0190] Table 17

[0191]

[0192] Remarks: 1) The interruption time is 205s; 2) The target fragmentation length is 150bp.

[0193] The quality inspection results after fragmentation are as follows: Figure 3-5 shown. in, image 3 is the fragmented quality inspection result of sample number 1, Figure 4 is the fragmented quality inspection result of s...

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Abstract

The invention relates to the field of gene mutation detection, in particular to a detection kit for NK / T cell lymphoma related genes and a library building method. The detection kit for the NK / T celllymphoma related genes comprises a capture probes for 65 target genes. The kit adopts two rounds of PCR reactions for library building, and the library building has a short period, a high coverage rate, a high comparison rate, a good uniformity, simple operations and the like.

Description

technical field [0001] The invention relates to the field of gene mutation detection, in particular to a NK / T cell lymphoma-related gene detection kit and a method for building a library. Background technique [0002] Lymphoma is a hematological malignancy originating from lymph nodes and lymphoid tissues, with complex pathological types and high incidence. Traditional lymphoma is mainly divided into two categories: Hodgkin's lymphoma (Hodgkin's lymphoma) and non-Hodgkin's lymphoma (NHL), which can be divided into B-cell NHL and T-cell NHL. category. NK / T-cell lymphoma (Natural-killer / T-cell lymphoma, NK / TCL) is a type of CD56-positive / CD3-positive lymphocyte malignant proliferation disease, which occurs in Asia and South America and is closely related to Epstein-Barr virus infection relevant. NK / TCL is highly malignant and aggressive, resistant to anthracycline-based chemotherapy regimens, and has a very poor clinical prognosis. About 75% of Ann Arbor stage I-II NK / TCL ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12Q1/6806
CPCC12Q1/6886C12Q1/6806C12Q2600/156C12Q2600/118C12Q2531/113C12Q2521/501C12Q2525/191
Inventor 赵维莅熊杰王楠付迪熊慧刘以哲俞浩崔帆
Owner RUIJIN HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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