Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A highly lytic Pseudomonas aeruginosa phage rdp-pa-20001 and its application

A technology of RDP-PA-20001 and Pseudomonas aeruginosa, applied in the direction of phage, virus/phage, application, etc., can solve the problems of ineffective medication and untimely treatment, and achieve strong lytic activity and wide acid-base tolerance range , the effect of good application prospects

Active Publication Date: 2021-11-19
RECOM QINGDAO BIOTECH CO LTD
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a highly lytic Pseudomonas aeruginosa phage RDP-PA-20001, which aims to solve the problem of acute onset of Pseudomonas aeruginosa infection in poultry farms, untimely treatment and drug resistance of pathogenic bacteria. Difficulties such as drug ineffectiveness due to sex

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A highly lytic Pseudomonas aeruginosa phage rdp-pa-20001 and its application
  • A highly lytic Pseudomonas aeruginosa phage rdp-pa-20001 and its application
  • A highly lytic Pseudomonas aeruginosa phage rdp-pa-20001 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Isolation and identification of Pseudomonas aeruginosa in BP-20001

[0030] Stool samples from farms with Pseudomonas aeruginosa bacterial disease, the method aseptic, were diluted 1:10 with LB broth sterilized taken 1:10 sample dilution was added to 10ml 90ml nutrient broth medium, cultured for 18-24 hours at 37 ℃. Surface of the growth medium with a thin film of bacteria, culture medium yellow-green.

[0031] Bacteria of the thin film enrichment broth cultures were picked, streaked on hexadecane bromide agar plates, placed in culture at 37 ℃ 18 ~ 24 hours, and appeared gray, flat, surface wetting, and the yellow-green of the surrounding agar colony, diameter of about 2 ~ 4mm. Typical continuously picked colonies streaked on hexadecane bromide agar purified three times, Individual colonies were picked and inoculated in 5ml of LB broth in a liquid medium, 37 ℃, 200rpm shaking 8h, to give uniformly turbid bacterial suspension. 16sRNA through the molecular identific...

Embodiment 2

[0032] Example 2 Isolation and identification of Pseudomonas aeruginosa phages RDP-PA-20001 of the embodiment

[0033] Experiments with the present invention is a stool sample collected in 2020 from a Jimo broiler farms stool pool, a phage separated sample.

[0034] (1) bacteriophage RDP-PA-20001 isolated:

[0035] Take 10ml LB broth stool 10㎎ disposed soaked overnight at 4 ℃, 10000rpm centrifuge 5min, the supernatant was sterilized with 0.22μm filter membrane. 3ml 3ml filtrate is taken and the host logarithmic phase bacterial culture was added to 20ml LB broth autoclaved together, placed in an incubator at 37 ℃ static overnight culture, there may be phage proliferation. A sample broth 3ml, 10000rpm centrifuge 5min, the supernatant was sterilized with 0.22μm filter membrane. Assuming that the above filtrate containing phage. Serially diluted with LB broth, prepared in a bilayer tablet logarithmic growth phase of the host growth medium, and cultured at 37 ° C and 19-23h. Appears on...

Embodiment 3

[0041] Example 3 morphological observation of phage

[0042] Using phosphate tungsungstic acid, 20 μl of phage proliferation solution (10) 9 PFU / ml) On the stone chilly tablets, the copper net was placed at 10 min to adsorb the phage. It was dried at 2-3 min at natural room, stained with 2% phosphotungstic acid hydrogen, and then suction moisture after 2 min, naturally dried 5 min, and observed the phage morphology in electrishry. Electron microscope photo ( figure 2 It can be seen that phage RDP-PA-20001 head is a noodle, and the stereo symmetry is 60.865 nm, the length of the phage is 183.254 nm, according to the latest classification and naming of the phage, belonging to the turtlee, the endococci .

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
lengthaaaaaaaaaa
Login to View More

Abstract

The invention discloses a highly lytic Pseudomonas aeruginosa phage RDP-PA-20001 and its application in poultry pathogenic Pseudomonas aeruginosa infection. The present invention uses a double-layer plate method to isolate a phage strain from the feces of a broiler chicken farm in Jimo, Qingdao, named Pseudomonas aeruginosa phage RDP-PA-20001, and the preservation number is CGMCC No.21410. Pseudomonas aeruginosa phage RDP-PA-20001 provided by the present invention has higher temperature tolerance and wider acid-base tolerance range, is conducive to industrialized production preparation; 20001 has a strong lysis effect and can effectively prevent and control the production and spread of Pseudomonas aeruginosa. This phage can be used as an efficient and reliable treatment for poultry and animal diseases caused by Pseudomonas aeruginosa.

Description

Technical field [0001] The present invention relates to the field of biotechnology, particularly to phage RDP-PA-20001 and its application Pseudomonas aeruginosa in a height lytic avian species Pseudomonas aeruginosa infection. Background technique [0002] Pseudomonas aeruginosa is a common gram-negative bacteria, Pseudomonas aeruginosa for short, is a very strong adaptability of bacteria, in addition to exist in the natural environment, but also in human skin, the intestinal tract, the respiratory tract the main cause nosocomial infections, often caused by respiratory, urinary tract infections and burn wounds. Shall not be detected bacteria in cosmetics hygiene standards, the pool of water pollution Pseudomonas aeruginosa can cause upper respiratory tract infection in people. Pseudomonas aeruginosa human consumption of contaminated food can cause food poisoning, foodborne Pseudomonas aeruginosa pollution has become one of the risk factors of harm to human health. Pseudomonas ae...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/00A01N63/40A01P1/00
CPCA01N63/40C12N7/00C12N2795/10321
Inventor 杜新永李先胜罗成盛赵丹丹王晓铃张庆王立坤刘玉庆盖春云马如霞刘爽
Owner RECOM QINGDAO BIOTECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com