Method for improving Anthurium andraeanum conversion efficiency
A technology of transformation efficiency and anthurium, which is applied in biochemical equipment and methods, horticultural methods, botanical equipment and methods, etc., can solve the problems of genetic transformation of anthurium stem segments, so as to improve the positive rate of plant transformation and reduce the Test cost, effect of inhibiting Agrobacterium contamination
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[0020] In order to clearly illustrate the technical features of the present solution, the present application will be described in detail below through specific implementation modes.
[0021] A kind of method of improving Anthurium transformation efficiency of the present invention, general steps are as follows:
[0022] S1. Acceptor material preparation;
[0023] Select 20-25d sterile Anthurium seedlings, cut the stems with axillary buds and pre-cultivate them as recipient materials; the pre-cultivation time is not less than 2 days;
[0024] S2. Bacterial solution preparation;
[0025] Use the plant expression vector pBI 121 with GFP gene, connect the chrysanthemum Flavanone3-hydroxylase gene by enzyme digestion, and then use the freeze-thaw method to introduce Agrobacterium E.coli DH5a to obtain the Agrobacterium with the plant expression vector pBI121-GFP-Flavanone 3-hydroxylase E.coli DH5a bacterial liquid;
[0026] Aspirate the bacterial liquid, transfer it to the LB l...
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