Method for remarkably improving instantaneous conversion efficiency of peach leaves through weak light treatment

Abstract

The invention belongs to the technical field of biology, relates to an instantaneous conversion method of peach leaves, and particularly relates to an instantaneous conversion method of peach seedlingleaves, and mainly solves the problem of low conversion efficiency of the peach leaves. The method comprises the following specific steps of: (1) removing external hard endocarps of peach pits storedin sand at a low temperature (4 DEG C), sowing seeds in nutrient soil, and culturing in an environment with the temperature of 22 DEG C, the humidity of 75% and the illumination of 6,000 to 8,000 lux; (2) culturing peach seedlings with the seedling age of 10 to 15 days for 5 to 8 days under the condition of the illumination intensity of 600 to 800 lux for later use; and (3) selecting the peach seedlings treated in the step (2), and performing instantaneous conversion by a conventional agrobacterium-mediated leaf vacuumizing method. The conversion efficiency of the peach seedling leaves subjected to weak light treatment is extremely remarkably improved; compared with a conventional conversion method, the method has the advantage that the GUS activity is improved by 5.64 times after GUS genes are instantaneously converted.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for instantaneous transformation of peach leaves, in particular to a method for instantaneous transformation of peach seedling leaves. Background technique [0002] Peach is one of the important deciduous fruit trees in my country. Its fruit is juicy and sweet, which is deeply loved by consumers and has extremely high economic value. With the completion of peach genome sequencing and the development of high-throughput sequencing technology, the research on important agronomic traits of peach has gone deep into the molecular level. Since peach has not yet established a mature and stable genetic transformation system, it is still a difficult problem for researchers to verify the function and regulation mechanism of peach genes. [0003] In the field of fruit tree research, the lack of a mature and stable genetic transformation system is a relatively common phenomenon, such as p...

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Problems solved by technology

[0005] The present invention proposes a high-efficiency instantaneous transformation method of peach le

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