A kind of black radish germplasm resource breeding and utilization method

A black radish and resource technology, applied in the field of breeding, can solve problems such as difficulty in utilization, and achieve the effects of improving utilization efficiency, reducing the risk of loss, and protecting intellectual property rights

CN112535099BActive Publication Date: 2022-07-08RICE & SORGHUM INST SICHUAN ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Publication Date
2022-07-08

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Abstract

The invention discloses a breeding and utilization method of black radish germplasm resources. Compared with radish Ogura CMS, the breeding of sterile lines is easier. All radish materials (including Ogura CMS hybrids) that do not contain orf463-specific genes can be bred into corresponding maintainer lines and sterile lines, and male-sterile hybrids can be easily prepared from NWB CMS sterile lines, which can be well protected for breeding. intellectual property rights. At the same time, various restorer lines created with the NWB CMS restorer gene contained in black radish can not only be used for the breeding of hybrid varieties, but also for the fixation and utilization of the excellent traits of NWB CMS hybrid sterile varieties on the market, reducing the loss of germplasm resources. risk and improve the utilization efficiency of germplasm resources.
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Description

technical field

[0001] The invention belongs to the technical field of breeding, in particular to a method for breeding and utilization of black radish germplasm resources. Background technique

[0002] my country is the largest producer of radish in the world, with an average annual planting area of ​​about 1.2 million hectares. Radish is a typical cross-pollinated plant with serious self-recession and obvious heterosis. The use of cytoplasmic malesterility (CMS) as the female parent for hybrid seed production is the main way to utilize the heterosis of radish. Cytoplasmic male sterility is a maternal inheritance caused by the combined action of cytoplasmic genes and nuclear genes. The intramolecular or intermolecular recombination or rearrangement of the mitochondrial genome can form chimeric genes or new open reading frames (ORFs) resulting in infertility. Fertility can be restored by the nuclear restorer gene (Restorer fertility, Rf).

[0003] In 1968, Japanese schola...

Examples

Embodiment

[0025] A method for breeding black radish germplasm resources, comprising the following contents.

[0026] (1) Materials to be tested

[0027] With three black radish germplasm resources imported from Europe ( figure 1 ) and 8 different types of radishes were used as test materials to carry out related research. The details of the materials are shown in Table 1.

[0028] Table 1 Details of the tested materials

[0029]

[0030]

[0031] (2) Material genomic DNA extraction and PCR amplification

[0032] The young leaves of radish were selected, and DNA extraction was carried out using the general plant DNA kit of Tiangen Bio (Beijing) Co., Ltd. (for the steps, refer to the kit instructions).

[0033] The primers were designed according to the specific gene orf463 sequence of the radish NWB CMS mitochondrial genome (Genebank ID: MN056360) published on the NCBI website. The sequence of the forward primer orf463-F is as follows:

[0034] GTGCCATTGCTGGAGTGAT (5'→3') (SEQ ...