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A cultivation method and technology of rhododendron orchid, applied in the field of high-yield rhododendron orchid seedling cultivation, can solve problems such as inability to obtain a large number of healthy rhododendron orchid seedlings, difficulty in rooting, deformed leaves of plants, etc.
Active Publication Date: 2021-05-07
湖北金水源农业开发有限公司 +2
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The inventor team used this method in practice and found that this method induces rapid germination and high proliferation rate of Rhododendron orchid protocorms, but there are disadvantages such as deformed plant leaves, abnormal pseudobulb expansion, and difficulty in rooting, and it is impossible to obtain a large number of healthy Rhododendron orchid seedlings
[0008] The rhododendron orchid seed germination, multiplica
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Embodiment 1
[0045] The present embodiment provides a kind of rhododendron bilobata seedling cultivation method, comprises the steps:
[0046] S1. Obtain sterile rhododendron orchid seeds that have been softened and sterilized.
[0047] This step specifically includes the following processes:
[0048] (1) Rinse the surface stains of Rhododendron bifoliate fruit pods (Rhododendron bilobata seed propagation material) collected at the domestication base with tap water, soak in laundry water for 20 minutes, gently scrub with a soft cloth, rinse with running water for 10 minutes, and dry in the air spare.
[0049] (2) Cut the dried rhododendron orchid fruit pods longitudinally with a scalpel to take out tiny rhododendron orchid double-leaf seeds, put an appropriate amount into a special seed disinfection bag made of non-woven fabric, and seal it with a sealing machine for later use.
[0050] (3) Slowly add 1mol / mL NaOH solution into 500mL distilled water to configure the testa softening solut...
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Abstract
The invention relates to a high-yield bifoliate cremastra appendiculata seedling cultivation method. The high-yield bifoliate cremastra appendiculata seedling cultivation method comprises the following steps of obtaining pure fruit pods of bifoliate cremastra appendiculata, carrying out softening treatment, disinfection and sterilization on testa of the bifoliate cremastra appendiculata to obtain sterile cremastra appendiculata seeds, sowing the sterile cremastra appendiculata seeds into a specific germination culture medium, carrying out germination culture to obtain light yellow protocorms, transferring the protocorms into a proliferation culture medium for proliferation culture to obtain green proliferation protocorms with white villus appearing on the proliferation surface of the green proliferation protocorms, transferring the green proliferation protocorms into a germination culture medium for differentiation germination culture to obtain rootless seedlings with expanded root pseudobulbs and differentiated clustered buds and a pair of leaves, and transferring the seedlings into a rooting culture medium for rooting culture to obtain tissue culture seedlings with expanded root pseudobulbs and 3-5 root hairs. By means of the cultivation method, a large number of high-yield bifoliate cremastra appendiculata domesticated seedlings can be obtained.
Description
technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for cultivating high-yield rhododendron bilobata seedlings. Background technique [0002] Rhododendron orchid, a perennial orchid herb, the pseudobulb is irregularly oblate or conical, the top gradually protrudes, and the base has fibrous root scars, 1.8 to 3 cm long, and 1 to 2 cm in diameter at the enlarged part. The leaves are usually 1, born on the top of the pseudobulb, narrowly elliptic, subelliptic or oblanceolate narrowly elliptic. Scapes emanate from upper nodes of pseudobulbs, nearly erect, racemes, somewhat drooping, not fully open, fragrant, capsules nearly oval, each containing 1200-2000 tiny seeds. The flowering period is from May to June, and the fruiting period is from September to December. Rhododendron orchid, as a plant in the family Orchidaceae, has many seeds, but the embryo is immature, lacks endosperm, double-layered seed coat, and t...
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