Micro-droplet generating device

Abstract

The invention provides a micro-droplet generating device. The micro-droplet generating device comprises a chip assembly, a pressing mechanism and a first driving mechanism, wherein the first driving mechanism drives the pressing mechanism to move in the vertical direction; the pressing mechanism comprises a puncture needle, a chip pressing plate, a first elastic piece and a second elastic piece; when the chip assembly moves to the position under the pressing mechanism, the pressing mechanism is pressed downwards, and air is communicated through the puncture needle to guide the air pressure into the chip assembly so as to generate micro-droplets. In the downward pressing process of the pressing mechanism, the pressing mechanism is firstly driven by the first driving mechanism, and then the pressing mechanism is driven by the elastic force applied by the first elastic piece to press the chip assembly, so that the functions of puncturing a silica gel film and pressing the microdroplet generation card are realized, and the microdroplet generation card can be protected from being crushed by rigid impact; in the process that the pressing mechanism leaves the chip assembly, the second elastic piece applies reverse elastic force to the chip pressing plate, and in the process that the puncture needle leaves the silica gel film, the chip pressing plate downwards presses the chip assembly, so that the microdroplet generation card is prevented from being taken up.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a microdroplet generating device. Background technique [0002] Digital PCR technology is a new nucleic acid detection and quantification method. It adopts an absolute quantitative method to directly detect the copy number of the target sequence without relying on the standard curve and reference samples. The principle of digital PCR: a standard PCR reaction is distributed into a large number of tiny reactors, and each reactor contains or does not contain one or more copies of the target molecule (DNA template) to achieve "single-molecule template PCR amplification". ”, after the amplification, the number of copies of the target sequence is “counted” by the number of positive reactors. The digital PCR process includes: droplet generation → film coating → amplification → droplet reading. Among them, droplet generation is used to generate thousands of droplets, which is realized usin...

AI Technical Summary

Problems solved by technology

The droplet generation device in the prior art is not fast enough to generate droplets, the negative pressure is unstable, the consistency of the generated droplets is uneven, and when the compression mechanism compresses the droplet generation card, it is easy to crush the droplet generation card

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