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A kind of rice resistance-related gene and its application

A rice and resistance technology, applied in the field of plant molecular biology and plant genetic engineering, can solve the problems of no rice black-streaked dwarf disease, few anti-virus genes and disease-resistance-related genes, etc., to improve disease resistance, enhance The effect of disease resistance

Active Publication Date: 2022-07-19
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few virus-resistant genes and disease-resistance-related genes in rice known so far, and there are no reports of rice black-streaked dwarf and southern rice black-streaked dwarf resistance-related genes

Method used

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  • A kind of rice resistance-related gene and its application
  • A kind of rice resistance-related gene and its application
  • A kind of rice resistance-related gene and its application

Examples

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Embodiment Construction

[0029] The following examples facilitate a better understanding of the present invention, but do not limit the present invention. The experimental methods in the following examples are conventional methods unless otherwise specified. The test materials used in the following examples were purchased from conventional biochemical reagent stores unless otherwise specified. The primers involved in the embodiments of the present invention are from Nanjing GenScript Biotechnology Co., Ltd.

[0030] 1. Preservation and culture of test plants and test viruses

[0031](1) The rice tested are NPB (Nipponbare) and W44 (VANDANA). W44 can be purchased from International Rice Research Institute (IRRI) and is the background plant of rice genetic transformation of the present invention. Among them, NPB is a susceptible variety of rice black-streaked dwarf virus and southern rice black-streaked dwarf virus, and W44 is a disease-resistant variety of rice black-streaked dwarf virus and southern...

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Abstract

The invention provides a rice resistance-related gene and application thereof, belonging to the field of plant molecular biology and plant genetic engineering. The invention provides application of a rice protein and its encoding gene: regulating the resistance of rice to rice black-streaked dwarf disease and southern rice black-streaked dwarf disease; breeding for rice black-streaked dwarf disease and southern rice black-streaked dwarf disease A rice variety with enhanced resistance to shrinkage disease; the amino acid sequence of the protein is shown in SEQ ID NO.1. The sequence of the coding gene is shown in SEQ ID NO.2. The OsAP47 gene of the invention negatively regulates the resistance of rice to rice black-streaked dwarf virus and southern rice black-streaked dwarf virus. The disease resistance of black-streaked dwarf virus, so as to achieve the purpose of increasing production and reducing drugs.

Description

technical field [0001] The invention belongs to the field of plant molecular biology and plant genetic engineering, in particular to a rice resistance-related gene and its application. Background technique [0002] Rice (Oryza sativa L.) is one of the most important food crops in the world and one of the most important cultivated crops in my country. . [0003] Rice black-streaked dwarf disease is a vicious viral disease transmitted by the vector striatellus planthopper in a persistent non-egg manner, which can infect rice, maize and wheat, causing rice black-streaked dwarf disease, maize rough dwarf disease and maize dwarf disease, respectively. Wheat green dwarf disease. Rice black-streaked dwarf virus (RBSDV) infection causes severe plant growth abnormalities, resulting in dwarf plants, failure to normal heading and fruiting, and losses of up to 50% after the onset of disease. Once a plant is infected, there is usually no cure, so it is called the "cancer" of rice. Ric...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/00A01H6/46
CPCC07K14/415C12N15/8283
Inventor 王招云周彤刘斌赵均良孙枫周炼周益军范永坚
Owner JIANGSU ACAD OF AGRI SCI
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