Manufacturing method of sediment paraffin section

A technology of paraffin section and production method, which is applied in the direction of instruments, measuring devices, scientific instruments, etc.

Active Publication Date: 2021-05-18
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

To date no studies have applied paraffin sectioning techniques to sediments as a medium

Method used

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  • Manufacturing method of sediment paraffin section
  • Manufacturing method of sediment paraffin section

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Experimental program
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Embodiment 1

[0044] The preparation method of the sediment paraffin section of embodiment 1 comprises the following steps carried out in sequence:

[0045] Step 1: Sampling, use a straw with a diameter of about 0.5 cm to insert a sediment columnar sample, and freeze it with liquid nitrogen for 10 minutes.

[0046] Note that the purpose of inserting the sediment columnar sample with a straw is to keep the original shape of the sample as much as possible, so as to facilitate subsequent serial sectioning and imaging observation.

[0047] Step 2: Fix, transfer the sample to a glass six-well plate, cut it gently and remove the straw, add 2.5% glutaraldehyde aqueous solution by volume fraction, and fix overnight in the dark.

[0048] Step 3: soaking, soaking the fixed sample three times with 0.1M PBS solution (pH=7.2), 10 min each time;

[0049] Step 4: dehydration, dehydration with gradient ethanol, sequentially dehydration treatment with 70% ethanol aqueous solution, 85% ethanol aqueous solut...

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Abstract

The invention discloses a manufacturing method of a sediment paraffin section. The method comprises the following steps: firstly, fixing a sediment sample by using glutaraldehyde, then carrying out gradient ethanol dehydration, transparent treatment, wax-dipping embedding, slicing and slice baking, rapidly treating the slice by using polyvinyl formal after slice baking, and purging a slice by using an ion humidifier, so that a layer of film can be formed on the surface of the slice, thereby protecting the details of the slice. The sediment slice cut by the method is complete in structure and clear in display, and has an obvious enhancement effect on observing the morphological structure and the like in the microorganisms.

Description

technical field [0001] The invention belongs to the field of making paraffin slices, in particular to a method for making sediment paraffin slices. Background technique [0002] Sediment is an important carrier of environmental information in water bodies, containing rich minerals, organic matter, and microorganisms. Microorganisms are the group with the largest biomass in sediments. Their morphology, structure and growth environment are important factors affecting their environmental ecological behavior. Therefore, in-situ observation of microorganisms in sediments is particularly important. Sediment slices can It is better to achieve this goal, but the relevant technology is relatively lacking. [0003] At present, the sediment section is mainly frozen section, but the current frozen section technology is mainly used in conventional animal and plant tissues, etc., because the adhesion of sediment particles is poor, which is far lower than that of conventional animal and p...

Claims

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): G01N23/2202G01N23/2251
CPCG01N23/2202G01N23/2251G01N2223/07G01N2223/102G01N2223/616
Inventor许玫英王斌黄友达
OwnerGUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY