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Pharmaceutical application of hydrogen sulfide synthase CSE

A technology for synthesizing enzymes and hydrogen sulfide, applied in the field of biomedicine, to achieve the effect of improving endothelial injury, reducing aortic aneurysm and aortic dissection

Inactive Publication Date: 2021-05-25
NANJING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, whether hydrogen sulfide changes during the occurrence of aortic aneurysm and aortic dissection, and whether its dysfunction is involved in the development of aortic aneurysm and aortic dissection, there are still no relevant studies

Method used

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  • Pharmaceutical application of hydrogen sulfide synthase CSE
  • Pharmaceutical application of hydrogen sulfide synthase CSE
  • Pharmaceutical application of hydrogen sulfide synthase CSE

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1. Western-Blot

[0019] (1) SDS-PAGE (polyacrylamide gel) electrophoresis: Prepare 12% separating gel and 3% stacking gel. Take 15 μL sample solution and 3 μL 6× loading buffer, and mix well. After boiling for 5 min to denature the protein, the sample was loaded with an amount of about 30 μg per well. Electrophoresis at a constant voltage of 110 V for about 90 min until bromophenol blue completely disappeared.

[0020] Preparation of SDS-PAGE:

[0021] Components Separating Gel (12%) Stacking Gel (3%)

[0022] Deionized water 1.6 mL 1.8 mL

[0023] 30% AA stock solution 2.0 mL 0.3 mL

[0024] 1.5M Tris-HCl (pH8.8) 1.3 mL ---

[0025] 1 M Tris-HCl (pH6.8) --- 0.75 mL

[0026] 10% SDS 50 μL 30 μL

[0027] 10% ammonium persulfate (APS) 50 μL 50 μL

[0028] TEMED 5 μL 5 μL

[0029] Total volume 5 mL 3 mL

[0030] (2) Membrane transfer: After electrophoresis, cut off the stacking gel, soak the gel in protein transfer buffer (3.6 g / L Tris, 300 mL / L methanol, 17.3 ...

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Abstract

The invention relates to pharmaceutical application of hydrogen sulfide synthase (CSE), in particular to application in preparation of drugs for treating aortic aneurysm and aortic dissection. The CSE adeno-associated virus improves the endothelial function and inhibits the onset of aortic aneurysm and aortic dissection. According to the invention, the effect of the hydrogen sulfide synthase CSE on endothelial injury caused by mechanical traction and prevention of aortic aneurysm and aortic dissection is defined. A new means is provided for treating the main aneurysm and the aortic dissection.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to the pharmaceutical use of hydrogen sulfide synthase CSE. Background technique [0002] Aortic aneurysm (AoA) and aortic dissection (AoD) are multifactorially induced and potentially fatal vascular diseases with a genetic predisposition. Aortic aneurysm manifests as aneurysmal dilation of the aorta, which is defined as dilation of the artery greater than 1.5 times the normal arterial diameter. Aortic dissection is manifested by tearing of the intima of the aortic wall, and circulating blood enters the media of the aortic wall through the intima breach, resulting in a large-scale tear of the media and the formation of a dissection. The etiology of more than 80% of patients with AoA and AoD is hypertension. According to statistics, AoA and AoD account for 1%-3% of the mortality rate of adult males aged 65-85 in developed countries. The pathological mechanisms of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/51A61K48/00A61P35/00A61P9/00
CPCA61K38/51A61K48/005A61P9/00A61P35/00C12Y404/01001
Inventor 季勇罗姗姗孔垂玉周雪纯
Owner NANJING MEDICAL UNIV
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