The invention belongs to the technical field of biological detection, relates to a new method for detecting the activity of
hydrogen sulfide synthase with a fluorescent probe of
hydrogen sulfide, and provides an application path of the method. It is suitable for detecting the activity of
hydrogen sulfide synthase including CBS, CSE and CL. For tissue samples, first
cut into small pieces and then
grind. For cells,
tissue cell lysate is used for lysing, and the lysate is used for
protein quantification. As shown in the figure, the
reaction system (
Tissue cell lysates, L-
cysteine, 5-
pyridoxal phosphate) were added to the reaction
pool on the outer ring of the reaction flask, and the absorption
system (H2S fluorescent probe working solution containing reaction
adjuvant alkane ammonium bromide) was added to In the absorption
pool in the inner ring, the H2S gas molecules produced by the
reaction system are combined with the fluorescent probes of the absorption
system, which can be used for
fluorescence measurement and
fluorescence imaging, quantitatively detect the H2S content, and more intuitively reflect the activity of H2S synthase. The invention can provide a good research method for studying H2S-related signaling pathways and the significance and diagnosis of H2S in some
disease processes.