Application of drug combination of vitamin K1 and temozolomide in preparation of medicine for treating cholangiocarcinoma
A technology of temozolomide and combined medication, which is applied in the field of medicine, can solve the problems that have not been reported yet, and achieve the effects of improving anti-tumor effect, reducing dosage, and reducing toxic and side effects
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0023] Example 1 Detection of the sensitivity of different cholangiocarcinoma cells to vitamin K1 by MTT method
[0024] 1. Experimental materials
[0025] (1) Drugs: vitamin K1 injection.
[0026] (2) Cholangiocarcinoma cells: human cholangiocarcinoma cells CCLP cells and HUCCT1 cells.
[0027] (3) A commercially available MTT kit.
[0028] 2. Experimental grouping
[0029] (1) Control group: blank control, that is, cholangiocarcinoma cells were not treated with any drug.
[0030] (2) Experimental group: cholangiocarcinoma cells were treated with different concentrations of vitamin K1.
[0031] 3. MTT assay to detect the sensitivity of different tumor cells to vitamin K1
[0032] (1) Human cholangiocarcinoma cell CCLP cells and HUCCT1 cells were seeded into 96-well plates at a number of 5000-9000 cells per well. After the cells adhered, PBS solutions of different concentrations of vitamin K1 were added respectively. After culturing for 24 hours, 10 μl of MTT at a concen...
Embodiment 2
[0034] Example 2 The effect of temozolomide alone or the combination of vitamin K1 and temozolomide on the inhibition rate of cholangiocarcinoma cells
[0035] 1. Experimental materials
[0036] (1) Drugs: vitamin K1 injection, temozolomide.
[0037] (2) Cholangiocarcinoma cells: human cholangiocarcinoma cells CCLP cells and HUCCT1 cells.
[0038] (3) A commercially available MTT kit.
[0039] 2. Experimental grouping
[0040] (1) Control group: Cholangiocarcinoma cells were treated with temozolomide only.
[0041] (2) Experimental group: Combined use of different concentrations of vitamin K1 and temozolomide to treat cholangiocarcinoma cells.
[0042] 3. MTT assay to detect the sensitivity of cholangiocarcinoma cells to the combination of vitamin K1 and temozolomide
[0043] (1) The human cholangiocarcinoma cells CCLP cells and HUCCT1 cells were seeded into 96-well plates at the number of 5000-9000 cells per well. After the cells adhered, PBS solutions with different rat...
Embodiment 3
[0045] Example 3 The effect of temozolomide alone or the combination of vitamin K1 and temozolomide on the inhibition rate of human cholangiocarcinoma tissue organoids
[0046] 1. Experimental materials
[0047] (1) Drugs: vitamin K1 injection, temozolomide.
[0048] (2) Cholangiocarcinoma cells: human cholangiocarcinoma tissue organoids.
[0049] (3) Commercially available CellTiter-Glo 3D kit.
[0050] 2. Experimental grouping
[0051] (1) Control group: only treated with temozolomide.
[0052] (2) Experimental group: different concentrations of vitamin K1 and temozolomide were used in combination to treat human cholangiocarcinoma tissue organoids.
[0053] 3. Sensitivity of human cholangiocarcinoma tissue organoids to the combination of vitamin K1 and temozolomide
[0054] (1) Mix the treated human cholangiocarcinoma cells with Matrigel, add 50 μl of mixed Mtrigel Matrigel to each well on a 48-well plate, add 250 μl of complete medium to each well after gel formation, ...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com