Transgenic component high-throughput screening method, detection kit and application thereof

Abstract

The invention discloses a transgenic ingredient high-throughput screening method, a detection kit and application thereof. The method comprises the following steps: according to the condition of exogenous insertion genes in commercialized transgenic crops, selecting 10 fragments capable of covering all commercialized transgenic strains as screening elements aiming at the exogenous genes, and obtaining conserved sequences of the 10 screening elements through bioinformatics analysis; a DNA primer pair designed aiming at a detection target and a corresponding TaqMan probe are embedded in each micropore of the microfluidic chip in advance, specific primers and probes are designed according to a sequence to carry out microfluidic chip detection, and whether a sample contains transgenic ingredients or not and which crop contains the transgenic ingredients are judged according to an amplification result. The sensitivity test of the method reaches 0.1%, and the method has good specificity. The kit and the detection method provided by the invention provide a good application prospect for high-throughput screening and detection of transgenic ingredients in prepackaged and processed food.

Description

technical field [0001] The invention relates to the technical field of gene detection, in particular to a high-throughput screening method for genetically modified components, a detection kit and applications thereof. Background technique [0002] In recent years, with the rapid development of transgenic technology, a large number of transgenic crops and their products have emerged. However, because the impact of genetically modified crops and their products on human health and biodiversity has not been tested for a long time, their safety has always been concerned by all walks of life. In order to protect consumers' right to know, right to choose and right to health of genetically modified products, various countries have established a variety of methods to detect genetically modified molecules in genetically modified plants and their products, in order to monitor the whole process of genetically modified plants from source to table. The European Union stipulates that when...

AI Technical Summary

Problems solved by technology

The ordinary PCR method has been gradually replaced by the real-time fluorescent PCR detection method due to its cumbersome operation and low sensitivity.

However, the detection cost of single-channel real-time fluorescent PCR method is high and the steps are cumbersome, so it is not suitable for multi-line screening detection of genetically modified products

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