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Medicine for treating renal tubule injury and application of medicine

A technology of renal tubules and drugs, applied in the field of medicine, to achieve the effect of inhibiting calcium oxalate stones and preventing oxidative damage

Pending Publication Date: 2021-06-15
杭州立效生物医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a kind of medicine and application thereof for the treatment of renal tubule injury, solve the problem of preventing RTECOI mentioned in the background technology

Method used

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  • Medicine for treating renal tubule injury and application of medicine
  • Medicine for treating renal tubule injury and application of medicine

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] cell culture

[0020] Renal Tubular Epithelial Cells The NRK-52E cell line is a kidney cell line of the Rattus norvegicus.

[0021] Cells were cultured in DMEM containing 10% fetal bovine serum and 1% penicillin-streptomycin.

Embodiment 2

[0023] In SM-L, SM-M and SM-H groups, NRK-52E cells were treated with 4μM, 6μM and 8μM solanine for 16 hours, and then treated with 0.75mM oxalate model, SM-L, SM-M and SM-H group for 3 hours; then immediately fixed with formalin for 15 minutes; after that, the sample was subjected to critical point drying and then coated with platinum; the coated sample was scanned using a cold-field scanning electron microscope, and the scanning electron microscope showed calcium oxalate crystals form.

[0024] attached figure 1 Middle arrows indicate calcium oxalate crystals.

[0025] see attached results figure 1 , solanine inhibits oxalate-induced deposition of calcium oxalate crystals in NRK-52E cells.

Embodiment 3

[0027] Flow Cytometry Analysis

[0028] NRK-52E cells were treated with 4μM, 6μM and 8μM solanine, and then treated with 0.75mM oxalate;

[0029] The method for detecting cell apoptosis is: centrifuge the cells at 4°C and 1000g for 5 minutes, and then incubate the obtained cell pellet with Annexin V-fluorescein isothiocyanate (FITC) and propidium iodide (PI) at room temperature 25 minutes; finally, the cell apoptosis rate was measured by flow cytometry.

[0030] see attached results figure 2 , 0.75mM oxalate significantly increased intracellular reactive oxygen species (ROS) levels and induced apoptosis; solanine concentration-dependently relieved the effect of oxalate.

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Abstract

The invention discloses a medicine for treating renal tubule injury and application of the medicine, and belongs to the technical field of medicines, and the concentration range of solamargine is 0-10 [mu] M; and the solamargine has the effect of protecting renal tubular epithelial cells from oxidative damage caused by kidney stones. By applying the solamargine, oxidative damage of renal tubular epithelial cells can be protected, and formation of oxalate calcium calculi is finally inhibited; and a candidate medicine is provided for preventing oxidative damage of the renal tubular epithelial cells.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a medicine for treating renal tubule injury and its application. Background technique [0002] Nephrolithiasis, also known as nephrolithiasis, is a very common urinary system disease affecting a large number of patients worldwide. Kidney stones are caused by an imbalance in the dissolution and precipitation of salts in the urinary tract and kidneys. Kidney stones are composed of 70% to 80% calcium oxalate, and high hematuria is a key risk factor for kidney stones. Its prevalence is estimated to be as high as 14.8% with an increasing trend, and the recurrence rate is as high as 50% within 5-10 years after the first episode. Risk factors such as obesity, diabetes, hypertension, and metabolic syndrome are important factors leading to the formation of KS. If not treated effectively, it can lead to significant morbidity and seriously affect the quality of life of patie...

Claims

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Application Information

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IPC IPC(8): A61K31/706A61P13/12
CPCA61K31/706A61P13/12
Inventor 吴晓凤
Owner 杭州立效生物医药科技有限公司
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