Medicine for treating renal tubule injury and application of medicine
A technology of renal tubules and drugs, applied in the field of medicine, to achieve the effect of inhibiting calcium oxalate stones and preventing oxidative damage
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0019] cell culture
[0020] Renal Tubular Epithelial Cells The NRK-52E cell line is a kidney cell line of the Rattus norvegicus.
[0021] Cells were cultured in DMEM containing 10% fetal bovine serum and 1% penicillin-streptomycin.
Embodiment 2
[0023] In SM-L, SM-M and SM-H groups, NRK-52E cells were treated with 4μM, 6μM and 8μM solanine for 16 hours, and then treated with 0.75mM oxalate model, SM-L, SM-M and SM-H group for 3 hours; then immediately fixed with formalin for 15 minutes; after that, the sample was subjected to critical point drying and then coated with platinum; the coated sample was scanned using a cold-field scanning electron microscope, and the scanning electron microscope showed calcium oxalate crystals form.
[0024] attached figure 1 Middle arrows indicate calcium oxalate crystals.
[0025] see attached results figure 1 , solanine inhibits oxalate-induced deposition of calcium oxalate crystals in NRK-52E cells.
Embodiment 3
[0027] Flow Cytometry Analysis
[0028] NRK-52E cells were treated with 4μM, 6μM and 8μM solanine, and then treated with 0.75mM oxalate;
[0029] The method for detecting cell apoptosis is: centrifuge the cells at 4°C and 1000g for 5 minutes, and then incubate the obtained cell pellet with Annexin V-fluorescein isothiocyanate (FITC) and propidium iodide (PI) at room temperature 25 minutes; finally, the cell apoptosis rate was measured by flow cytometry.
[0030] see attached results figure 2 , 0.75mM oxalate significantly increased intracellular reactive oxygen species (ROS) levels and induced apoptosis; solanine concentration-dependently relieved the effect of oxalate.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com