Amino acid high-throughput target detection method and application thereof

A detection method and high-throughput technology, applied in the field of amino acid detection, can solve problems such as different contents, and achieve the effects of simple operation, short processing period and short analysis time.

Pending Publication Date: 2021-06-22
上海阿趣生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, since there are many types of amino acids and their contents are also different, there are many difficulties in the analysis of amino acid metabolites. Among them, how to efficiently and accurately measure the metabolites of amino acids while increasing the throughput is one of the problems that need to be solved at present. The present invention Provide an amino acid high-throughput target detection method to accurately, efficiently and sensitively detect amino acid metabolites while ensuring coverage of multiple amino acids

Method used

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  • Amino acid high-throughput target detection method and application thereof
  • Amino acid high-throughput target detection method and application thereof
  • Amino acid high-throughput target detection method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0093] A high-throughput target detection method for amino acids, comprising the following steps: S1: extraction of metabolites; S2: configuration of standard solutions; S3: on-machine detection and analysis.

[0094] The step S1:

[0095] 1) Put 21.6mg of the sample to be tested in an EP tube, add 1000ul of extract, and vortex to mix evenly;

[0096] 2) Perform homogenization and ice-water bath ultrasonication, and repeat the homogenization and ultrasonication steps to obtain the sample liquid;

[0097] 3) leave the sample liquid still;

[0098] 4) centrifuging the sample solution after standing;

[0099] 5) Take the supernatant to an LC injection vial for UHPLC-MS / MS analysis.

[0100] The sample to be tested is rice flour.

[0101] The extracting liquid is configured as acetonitrile:methanol:water=2:2:1 by volume.

[0102] The extract should be pre-cooled at -20°C.

[0103] The extract contains an isotope internal standard mixture.

[0104] The number of times for repe...

Embodiment 2

[0129] A high-throughput target detection method for amino acids, the specific implementation is the same as in Example 1, except that the homogenization frequency is 45 Hz.

Embodiment 3

[0131] A high-throughput target detection method for amino acids, the specific implementation is the same as in Example 1, except that the temperature of the column oven is 38°C.

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Abstract

The invention provides an amino acid high-throughput target detection method and application thereof. The method specifically comprises the following steps: S1, extracting metabolites; S2, preparing a standard solution; and S3, detecting and analyzing on a machine. The amino acid high-throughput target detection method is high in sensitivity, and can accurately identify a plurality of different amino acids and accurately determine the component content of the amino acids at the same time.

Description

technical field [0001] The invention relates to the technical field of amino acid detection, in particular to a high-throughput target detection method for amino acids and its application. Background technique [0002] In order to survive, human beings must ingest food and maintain the normal metabolic activities of the body. Protein is the main carrier of biological functions and also the nutrient substance needed by the human body. Amino acids are the building blocks of proteins, so amino acids participate in multiple metabolic pathways and play a key role. Judging the metabolism of amino acids by analyzing the content of amino acids can help prevent and diagnose many diseases. It can not only protect human health, but also play an important role in regulating the orderly production and metabolism of various organisms. [0003] However, since there are many types of amino acids and their contents are also different, there are many difficulties in the analysis of amino ac...

Claims

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/72
CPCG01N30/02G01N30/06G01N30/72G01N2030/045
Inventor楼格尔周敏张昆黄明旺王雪凌加焱周晓青王洪光
Owner上海阿趣生物科技有限公司