Serum lipid metabolite composition, kit and application
A kit and marker technology, applied in clinical medicine, medicine, and analytical chemistry, to achieve the effect of reducing lung cancer mortality, high sensitivity, and good application prospects
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0024] 1. Collection of Serum Samples
[0025] Before sampling, all volunteers included in the study signed the informed consent. Fasting serum samples from 63 female healthy controls and 64 lung cancer patients (including non-smoking females with stage 0, stage I, stage II and stage III-IV lung cancer) were collected. All subjects fasted for more than 8 hours, and fasting blood was collected. After blood collection, the serum was separated and stored in a -80°C refrigerator.
[0026] 2. Analysis method
[0027] 2.1 Pretreatment of serum samples
[0028] Thaw the blood sample at 4°C, take 40 μL serum sample, add 300 μL methanol solution containing internal standard 6.7 μg / mL palmitic acid-16,16,16-d 3 and 3.3 μg / mL phosphatidylethanolamine PE15:0 / 15:0 , then rotate for 1 min, and then add 1 mL of methyl tert-butyl ether to fully extract the lipids. Then 300 μL of water was added for phase separation. The mixture was centrifuged at 10000g, 4°C for 10 minutes. The superna...
PUM
Property | Measurement | Unit |
---|---|---|
Sensitivity | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com