Purification Amplification Device

Abstract

The invention relates to a purification and amplification device, which includes: a substrate and a pipeline structure arranged on the substrate, the pipeline structure includes several liquid inlets, PCR amplification chambers and purification chambers; the liquid inlet includes a first A sample inlet, a first reagent port, a second reagent port, a third reagent port and a fourth reagent port, the first sample inlet and the first reagent port are connected through a first pipeline, the purification chamber includes an inlet and an outlet, the second A sample inlet is connected to the inlet through a second pipeline, the first reagent port is connected to the outlet through a third pipeline, and the second reagent port, the third reagent port and the fourth reagent port are all connected to the inlet through a fourth pipeline; The first end of the PCR amplification chamber is provided with the first part of the double valve, the second end of the PCR amplification chamber is provided with the second part of the double valve, and the first part of the double valve passes through the fifth pipeline and the first single valve connection, the first single valve is connected to the outlet through the sixth pipeline; the purification and amplification device provided by the present invention does not need to add electrodes in the pipeline structure to realize the extraction of amplified substances.

Description

technical field [0001] The invention relates to the field of nucleic acid detection, in particular to a purification and amplification device. Background technique [0002] In scientific experiments such as biology, chemistry, and materials, it is often necessary to operate on fluids, such as the preparation of sample DNA, liquid chromatography, PCR reaction, and electrophoresis detection, all of which are performed in a liquid-phase environment. If the steps of sample preparation, biochemical reaction, and result detection are to be integrated on the biochip, the amount of fluid used in the experiment will be reduced from milliliters and microliters to nanoliters or picoliters. At this time, powerful microfluidic devices are necessary. Indispensable. Therefore, with the development of biochip technology, microfluidic technology, as a key supporting technology of biochip, has also received more and more attention. [0003] The purification and recovery of nucleic acid is a...

AI Technical Summary

Problems solved by technology

The combination of pipeline structure and electrodes is used, not only the complex structure makes the production of the pipeline layer more difficult, but also the setting of the electrodes is cumbersome, there are many factors to consider, and it is not easy to realize

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