36results about How to "Reduce the difficulty of experimental operation" patented technology

The invention discloses a tangential and normal resistance testing device and method for anchor chain and soil under an equivalent elastic boundary. The device comprises a supporting rack, a transmission device, an air pressure loading system, a test box, a vertical displacement applying part, a tangential anchor chain unit, a normal anchor chain unit and the like. By employing an equal rigidity spring to simulate the elastic boundary, the size of the test box can be reduced greatly in the condition that the boundary condition is affected, so that the materials can be saved effectively and theexperimental operating difficulty can be reduced effectively. Meanwhile, the device can integrate tangential and normal resistance tests, can measure the tangential and normal interactions of the anchor chain and the soil, and can provide important design parameters for calculating a buried fragment of an engineering anchor chain. The device and method have important reference meaning in a mooring system of an anchor point below the sea bottom mud face.

The invention belongs to the technical field of chemical catalysis, and particularly relates to a preparation method of a high-activity electrochemical oxygen reduction catalyst. The preparation method comprises the following steps: ultrasonically stirring and dissolving a phthalocyanine material in an organic solvent, loading the phthalocyanine material on a specially treated conductive materialin an organic matter leaching manner, filtering, and cleaning to obtain the high-activity electrochemical oxygen reduction catalyst. According to the invention, the preparation method of the high-activity electrochemical oxygen reduction catalyst is developed, and the obtained electrochemical oxygen reduction catalyst has excellent electrochemical performance; the method is low in raw material cost, simple in reaction process, low in experimental operation difficulty, good in experimental repeatability and free of additives difficult to remove; and the prepared high-activity electrochemical oxygen reduction catalyst has high electrochemical oxygen reduction efficiency and good electrochemical stability, and can achieve the half-wave potential of 0.9 V compared with the traditional commercial Pt/C (0.86 V) under the same condition.

The invention relates to an emulsion droplet digital PCR quantitative method based on microspheres and microcolumn array chips. The emulsion droplet digital PCR quantitative method comprises the steps that firstly, the microcolumn array chips are prepared; secondly, polystyrene microspheres with the surfaces modified with streptavidin are extracted, diluted and then added into turbid liquid of target magnetic beads step by step for incubation, and a microsphere and magnetic bead composite is enriched and resuspended; thirdly, a resuspended solution of the microsphere and magnetic bead composite in the second step is taken and introduced into a device to be washed, the microsphere and magnetic bead composite intercepted on the chips is counted, and the number of the target magnetic beads is obtained, so that the purpose of high-sensitivity quantification on nucleic acid is achieved. According to the emulsion droplet digital PCR quantitative method based on the microspheres and the microcolumn array chips, the chips are easy to assemble and low in cost; the target magnetic bead counting method of a BEAMing technology is more convenient and easier to implement while the advantage of high sensitivity of a BEAMing experiment is maintained, and a practical tool easy, convenient and fast to use is provided for high-sensitivity detection of nucleic acid.

The invention discloses a multi-component alloy diffusion couple device and a multi-component alloy diffusion coefficient determination experiment method, a diffusion couple comprises a counterweightmodule and three cylindrical refractory materials with blind holes, and the blind holes are all located in the centers of the refractory materials. The blind hole of the refractory material at the upper end is used for accommodating a solid material I for preparing the diffusion couple; wherein the blind hole with larger diameter at the upper end in the refractory material at the lower end is usedfor collecting redundant melt after the interface of the solid material I and the solid material II is attached, the blind hole with smaller diameter at the lower end accommodates the refractory material with smaller diameter and blind hole, the refractory material accommodates the solid material II, and the solid material I is in contact with the solid material II. The three refractory materialsare sequentially filled into a quartz tube for vacuum sample sealing treatment, so that the oxidation pollution of the sample is reduced. According to the device and the method, the thermal diffusionand interface reaction conditions of the melt interface under the vacuum condition can be obtained, the process cost is low, the operation is simple, and the stability is good.

The invention discloses a non-contact strain field and acoustic emission combined split type measuring system and method. The system comprises a testing sample, two testing machine clamps, an acoustic emission sensor, an acoustic emission acquisition system, a camera, image acquisition control equipment and a fixing device, wherein the testing sample adopts a rectangular strip nonmagnetic material; the two testing machine clamps respectively clamp the upper and lower ends of the testing sample and stretch the testing sample; the acoustic emission sensor and the camera are respectively fixed on the left and right opposite sides of the testing sample; the acoustic emission sensor sends collected acoustic signals to the acoustic emission acquisition system; the camera sends collected image signal to the image acquisition control equipment; the acoustic emission sensor adopts a cylinder; a sensing surface is formed on the top surface of the cylinder; the acoustic emission sensor is fixed through the fixing device, so as to ensure that the sensing surface of the acoustic emission sensor is adhered to the surface of the testing sample. The system is simple in structure, and high in reliability and testing precision, and can effectively solve the problem in fixing the nonmagnetic material.

The invention discloses an adjustable microorganism cultivation dish operating frame. The adjustable microorganism cultivation dish operating frame is characterized in that a lifting frame is arranged at the upper end of a cultivation dish operating frame base, a left lifting support is arranged at the left end of the lifting frame, a right lifting support is arranged at the right end of the lifting frame, a lifting rack is arranged in the middle of the lifting frame, and an operating worktable is arranged at the upper end of the left lifting support and the upper end of the right lifting support and is fixedly mounted at the upper end of the left lifting support, the upper end of the right lifting support and the upper end of a connecting fixing plate. A transmission shaft is arranged between left and right fixing frames, a lifting gear is arranged in the middle of the transmission shaft and is mounted on the front side of the lifting rack, and a lifting adjusting handle is arranged at the left end of the transmission shaft. A mixing dish shaking frame is arranged on the right side of the lifting frame, a stationary solidification frame is arranged on the right side of the mixing dish shaking frame, and a stationary worktable is arranged at the upper end of the stationary solidification frame.

The invention discloses a power electronic integrated module layered cold plate performance test platform and test method, the test platform comprises a rack, a cold plate is connected to the rack, the inlet of the cold plate is connected with the outlet of a water storage tank sequentially through an inlet water temperature control module, a flow controller and a water pump, and the water storagetank, the water pump, the flow controller and the inlet water temperature control module form a cooling liquid supply module; a cold plate outlet is connected with a water return port of the water storage tank; a thermal load applying module is arranged on the cold plate and is provided with a heat source temperature measuring part; an inlet temperature measuring part is arranged at the inlet ofthe cold plate, and an outlet temperature measuring part is arranged at the outlet of the cold plate; an environment temperature measuring part is arranged on the rack; the environment temperature measuring component, the outlet temperature measuring component, the inlet temperature measuring component and the heat source temperature measuring component form a temperature measuring module; a pressure difference measuring module is connected between the inlet and the outlet of the cold plate; according to the invention, heat loss measurement and heat dissipation performance measurement of different sizes and structures are realized on the test platform.

The invention discloses a real-time fluorescent PCR method for detecting mycobacterium avium (MA) and an application of primer pairs. The invention provides the specific primer pairs, and establishesthe real-time fluorescent PCR method for detecting the MA based on SYBR Green I fluorescent dye. The method has high sensitivity, good specificity and good reproducibility, and has the advantages of acheaper price and easier operation compared with those of methods such as a DNA chip technology and a PCR-fingerprint; compared with a current ''gold standard'' of strain identification, the method has no significant differences for detecting the MA, and the simple, cheap, and highly accurate molecular biology method is provided for clinical identification of the MA; the method improves the workefficiency of inexperienced experimental technicians, reduces the difficulty of experimental operation, and reduces the probability of errors, so that the experimental technicians can perform experiments after being trained; and compared with other molecular biology methods, in the case of having higher diagnostic accuracy, the method provided by the invention reduces costs, reduces experimental funds, and is easier to promote clinically.

The invention relates to a pretreatment method for detecting the cell breakage rate of rolled leaves. The method comprises the following steps of 1) taking the rolled leaves, and carrying out water bath treatment for 1-5 minutes at the temperature of 80-100 DEG C; 2) spreading the treated rolled leaves, wiping off redundant water on the surfaces of the rolled leaves, and then flatly laying the rolled leaves on a photographing background; and (3) photographing the rolled leaves under a stable light source. According to the method disclosed by the invention, water bath treatment is adopted, and tea polyphenol overflowing from broken cells can be promoted to be oxidized at proper treatment temperature and treatment time, so tea pigment is formed, and the tea pigment is used for judging the broken cells of the leaves; secondly, after the pretreatment, computer analysis is adopted to replace manual estimation to obtain a cell disruption rate result. The method disclosed by the invention is simple and controllable, errors caused by human factors in an experiment can be reduced, accuracy of measuring the cell breakage rate is improved, the analysis speed is increased, and working efficiency is improved.