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Screening method of termite-source forage bacillus

A Bacillus and screening method technology, which is applied in the field of screening of termite-derived feedable Bacillus, can solve the problems of heavy re-screening workload, high cost, and difficult operation, and achieve reduced re-screening workload, improved efficiency, and reduced The effect of screening costs

Pending Publication Date: 2021-10-01
HAINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This screening method is difficult to operate, low in efficiency, heavy in workload and high in cost for re-screening

Method used

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  • Screening method of termite-source forage bacillus
  • Screening method of termite-source forage bacillus
  • Screening method of termite-source forage bacillus

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Embodiment

[0029] In this embodiment, the medium formula used is as follows:

[0030] i. Primary screening medium: CMC-Na 10.0g, peptone 2.0g, K 2 HPO 4 4.0g, (NH 4 ) 2 SO 4 2.0g, MgSO 4 ·7H 2 O 0.3g, agar 15.0g, dilute to 1000mL with distilled water, pH=6.5.

[0031] ii. Re-screening medium: CMC-Na 10.0g, peptone 2.0g, K 2 HPO 4 4.0g, (NH 4 ) 2 SO 4 2.0g, MgSO 4 ·7H 2 O 0.3g, Congo red 0.4g, agar 15.0g, dilute to 1000mL with distilled water, pH=6.5.

[0032] iii. Fermentation enzyme production medium: CMC-Na 10.0g, peptone 2.0g, K 2 HPO 4 4.0g, (NH 4 ) 2 SO 4 2.0g, MgSO 4 ·7H 2 O 0.3g, dilute to 1000mL with distilled water, pH=6.5.

[0033] iv. DNS solution: KNaCHO 6 4H 2 O 182.0g, NaOH 20.96g, DNS 6.3g, PhOH 2.5g, NaOH 2 SO 3 5.0g. Dilute to 1000mL with distilled water.

[0034] A kind of screening method of feedable bacillus provided by the present embodiment mainly comprises the following steps:

[0035] (1) Grinding termites: first wash the termites ...

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Abstract

The invention provides a screening method of termite-source forage bacillus, and the method comprises the following steps: adding normal saline, grinding termites after body surface disinfection into liquid, treating the grinding liquid in a heat treatment manner, and performing screening by a selective culture medium to obtain a high-yield cellulase strain; and identifying and analyzing the strain to obtain the bacillus capable of being used for feeding. According to the method disclosed by the invention, the experimental operation difficulty of picking away from the intestinal tract of the termite is reduced, strains with strong functionality are easier to screen out, the secondary screening workload is greatly reduced, the bacterium screening efficiency is remarkably improved, and the bacterium screening cost is reduced.

Description

technical field [0001] The invention belongs to the field of animal nutrition and feed science, and relates to the screening and application of cellulase-producing strains, in particular to a screening method for termite-sourced feedable bacillus. Background technique [0002] In China's agricultural production, a large amount of cellulose has not been efficiently utilized, and cellulose itself is an important component of ruminant feed, so the research on its degradation and utilization is of great significance. In the natural environment, the existing form of cellulose is mainly combined with hemicellulose and lignin to form lignocellulose. The structural characteristics of cellulose from different sources vary greatly, including crystalline regions with high stability and low degradation, and amorphous regions with low stability and relatively easy degradation. The crystalline region and the amorphous region form a complex and dense structure through mixing and crosslink...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C12R1/07
CPCC12N1/20C12N1/02
Inventor 何晓涛陈杰茹韩蒙蒙杨雨辉朱昊多水银韦起壮杨兆裕陈韬宇
Owner HAINAN UNIVERSITY
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