Living cell screening method based on high-throughput antibody chip

Abstract

The invention provides a living cell screening method based on a high-throughput antibody chip. The method comprises the steps of antibody chip point preparation, living cell quality inspection, chipsealing, living cell collection, living cell dyeing, living cell and antibody chip incubation, chip cleaning and airing, chip scanning, data processing and the like. With reaction of the antibody chipand the dyed living cells, the antibody capable of being combined with the cell surface target can be selected through screening in a short time, the experimental operation difficulty is low, the applicability is wide, various samples capable of being prepared into the single-cell suspension can be suitable for the method, and the cost is greatly reduced compared with that of a traditional targetdevelopment method.

Description

Technical field[0001]The present invention relates to the field of biotechnology, in particular to a method of screening live cell high-throughput based on an antibody chip.Background technique[0002]Screening process in living cells, drug targets involved in receptor, enzyme, ion channel, transporter, immune system genes, with respect to the drug target is unknown, a known drug target just the tip how can discover and find an effective drug targets in many of today's problems is unknown in the field of medicine, the development of new targets plays a vital role in the drug development process, the modern pharmaceutical research, new targets The establishment of new drugs is often the prerequisite and guarantee for new drug innovation. At present, most pharmaceutical companies lack effective means for the development of new targets. At present, the number of targets discovered by humans has stagnated at about 500. The cycle of discovery and confirmation of new targets is generally Ab...

AI Technical Summary

Problems solved by technology

[0002] In the screening process of living cells, drug targets involve receptors, enzymes, ion channels, transporters, immune system, genes, etc. Compared with unknown drug targets, known drug targets are just the tip of the iceberg , how to discover and find effective drug targets in many unknowns is a difficult problem in the field of medicine today. The development of new targets plays a vital role in the entire drug research and development process. In modern drug research, new targets The establishment of new drug innovation is often the premise and guarantee of new drug innovation. However, most pharmaceutical companies currently lack effective means for the development of new targets. At present, the number of targets discovered by humans has stagnated at about 500. The cycle of discovery and confirmation of new targets is generally within About three to five years, not only the cycle is long, the reproducibility and throughput are low, but also the development of antibodies requires follow-up verification, and the development cost is high