Purification amplification device

Abstract

The invention relates to a purification amplification device, which comprises a base plate and a pipeline structure arranged on the base plate, and the pipeline structure comprises a plurality of liquid inlets, a PCR amplification bin and a purification bin; the liquid inlet comprises a first sample inlet, a first reagent port, a second reagent port, a third reagent port and a fourth reagent port, the first sample inlet and the first reagent port are connected through a first pipeline, the purification bin comprises an inlet and an outlet, the first sample inlet and the inlet are connected through a second pipeline, the first reagent port and the outlet are connected through a third pipeline, and the second reagent port, the third reagent port and the fourth reagent port are connected with the inlet through a fourth pipeline; the first end of the PCR amplification bin is provided with a first part of the double valves, the second end of the PCR amplification bin is provided with a second part of the double valves, the first part of the double valves is connected with the first single valve through a fifth pipeline, and the first single valve is connected with the outlet through a sixth pipeline; according to the purification and amplification device provided by the invention, an electrode does not need to be added in a pipeline structure, and amplification substances are extracted.

Description

technical field [0001] The invention relates to the field of nucleic acid detection, in particular to a purification and amplification device. Background technique [0002] In scientific experiments such as biology, chemistry, and materials, it is often necessary to operate on fluids, such as the preparation of sample DNA, liquid chromatography, PCR reaction, and electrophoresis detection, all of which are performed in a liquid-phase environment. If the steps of sample preparation, biochemical reaction, and result detection are to be integrated on the biochip, the amount of fluid used in the experiment will be reduced from milliliters and microliters to nanoliters or picoliters. At this time, powerful microfluidic devices are necessary. Indispensable. Therefore, with the development of biochip technology, microfluidic technology, as a key supporting technology of biochip, has also received more and more attention. [0003] The purification and recovery of nucleic acid is a...

AI Technical Summary

Problems solved by technology

The combination of pipeline structure and electrodes is used, not only the complex structure makes the production of the pipeline layer more difficult, but also the setting of the electrodes is cumbersome, there are many factors to consider, and it is not easy to realize

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