FibroTouch-based non-alcoholic fatty liver disease rat model and manufacturing and detection methods thereof

A fatty liver disease, rat model technology, applied in the field of medical testing and testing, can solve the problems of difficulty in large-scale development, invasive operation, high cost, etc., and achieve the effect of deepening the review and comparison analysis, obvious application value, and intervening in the progress of the disease.

Inactive Publication Date: 2021-07-30
邵建国
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the diagnosis of NAFLD in many areas still mainly relies on invasive liver biopsy. However, due to factors such as the large number of patients, invasive procedures, and high costs, it is difficult to carry out large-scale implementation in China, which brings considerable benefits to the diagnosis of NAFLD. Therefore, in order to solve such problems, we propose a rat model of non-alcoholic fatty liver disease based on FibroTouch and its production and detection methods

Method used

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  • FibroTouch-based non-alcoholic fatty liver disease rat model and manufacturing and detection methods thereof
  • FibroTouch-based non-alcoholic fatty liver disease rat model and manufacturing and detection methods thereof
  • FibroTouch-based non-alcoholic fatty liver disease rat model and manufacturing and detection methods thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] S1: Sampling marking: Liver sampling is performed for each individual rat individual in the normal feeding group and the sample feeding group, and marked according to the individual number of the sample;

[0035] S2: Fibrotouch technology detection: use Fibrotouch technology to detect each liver sample, and detect LSM value and UAP value;

[0036] Further explanation, in the S1 part, with the conversion coefficient of 0.018 as the conversion unit, the test display value = the actual value of the rat / 0.018;

[0037] In step S2, LSM value 17.5kPa is defined as liver cirrhosis decompensation stage (F4 ), UAP value 295dB / m was defined as severe fatty liver (grade 3).

[0038] Detect the LSM value and UAP value of each individual rat, and generate the table as follows:

[0039]

[0040] and combine figure 2 , draw the following conclusions: According to the scatter diagram of the patient's LSM and UAP values, it is found that the scatter points of the LSM and UAP va...

Embodiment 2

[0041] In embodiment two: different from embodiment one:

[0042]S3: Biochemical index detection: perform biochemical index detection on the liver samples in S2, and the detection direction of each liver sample corresponds to the LSM value and UAP value. The biochemical index detection parameters include ALT, AST, creatinine, TC, TG, HDL- C, LDL-C, fasting blood sugar and BMI;

[0043] S4: Parameter data analysis and comparison: Combine the LSM value, UAP value and biochemical index detection parameters in S3 to create an independent table, and calculate the median value according to statistics.

[0044] Generate the following tables based on the data:

[0045]

[0046]

[0047]

[0048]

[0049] In summary: Combining the values ​​of multiple tables, the effective rate of Fibrotouch detection and diagnosis of liver fibrosis is 87.5%, and the effective rate of detection and diagnosis of fatty liver is 92.8%, which has a high detection efficiency. By comparing the L...

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Abstract

The invention discloses a FibroTouch-based non-alcoholic fatty liver disease rat model and manufacturing and detecting methods. The FibroTouch-based non-alcoholic fatty liver disease rat model comprises a plurality of mature rat individuals, and is characterized in that the selected rat individuals are bred in the same batch and have the same breeding condition, the body lengths and the body weights of the selected rat individuals are roughly the same, the selected rat individuals are healthy and free of diseases, the conversion coefficient of the body weight coefficient is 0.018, the body weight coefficient is equal to (Kg)/height (m) 2), the conversion coefficient is equal to (the body weight coefficient of the rat individuals/the body weight coefficient of the adult group), and the selected rat individuals are grouped according to male and female. According to theFibroTouch-based non-alcoholic fatty liver disease rat model and the manufacturing and detecting methods provided by the invention, the LSM and UAP values of an NAFLD patient are detected through Fibrotouch, and indexes such as liver function, blood fat and blood sugar, BMI and the like are combined, so that the liver fibrosis and lipolysis lesion degrees of the NAFLD patient are more accurately judged, the positive intervention of disease progression is facilitated, and the application value is obvious in clinic use.

Description

technical field [0001] The invention relates to the technical field of medical detection and testing, in particular to a FibroTouch-based rat model of nonalcoholic fatty liver disease and a method for making and detecting it. Background technique [0002] Non-alcoholic fatty liver disease (NAFLD) refers to a clinicopathological syndrome characterized by excessive fat deposition in liver cells caused by alcohol and other definite liver-damaging factors, and is closely related to insulin resistance and genetic susceptibility. Metabolic stress-induced liver injury. Including simple fatty liver (SFL), nonalcoholic steatohepatitis (NASH) and related liver cirrhosis, the prevalence of NAFLD in ordinary adults is 10% to 30%, of which 10% to 20% are NASH, the latter within 10 years. The incidence of cirrhosis is as high as 25%, and the harm of non-alcoholic fatty liver disease to human health will continue to increase; [0003] At present, the diagnosis of NAFLD in many areas stil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/027G01N33/50
CPCA01K67/027G01N33/5088A01K2207/25A01K2227/105A01K2267/0362A01K2267/02
Inventor 邵建国陈琳吉祥居林玲曹亚丽罗蕾蕾周倩罗茜王慧璇何红梅卞兆连徐姝嫣
Owner 邵建国
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