Human heparin binding protein immunoturbidimetry detection reagent and application thereof

Abstract

The embodiment of the invention discloses a kit for detecting heparin binding protein in a sample. The kit comprises a pretreatment reagent for pretreating a sample and a recognition reagent for recognizing heparin binding protein. The pretreatment reagent comprises a hemolytic agent and an electrolyte, the hemolytic agent is TritonX-100 with the concentration of 1-3 mg / mL, the electrolyte is sodium chloride with the concentration of 30-60 mg / mL, and the pH value of the pretreatment reagent is 5.5-6.5. The kit can be used for detecting the concentration of the heparin binding protein by using a whole blood sample, the blood sampling amount required for detection is small, and the detection time is short. The embodiment of the invention further discloses application of the kit in quantitative detection of the heparin binding protein and a method.

Description

technical field

[0001] This specification relates to the field of immunoassay, in particular to a human heparin-binding protein immunoturbidimetric detection reagent and its application. Background technique

[0002] Heparin-binding protein (HBP), also known as CAP37 or azurecin, is a granular protein with various functions released by neutrophils upon external stimulation. HBP can activate monocytes and macrophages, has significant antibacterial activity, chemotactic properties, and promotes vascular leakage, making it an emerging infectious disease-related biomarker. Once a bacterial infection occurs, the infection-related M protein combines with fibrinogen, resulting in the activation of multilineage leukocytes and the secretion of a large amount of HBP. HBP is widely used in the diagnosis and differential diagnosis of clinical infectious diseases, especially in the early diagnosis and curative effect monitoring of patients with sepsis, and also in other infectious disea...

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