Reagent and detection method for detecting D614G mutant of SARS-COV-2 virus

A SARS-COV-2, D614G technology, applied in the field of genetic testing, can solve problems such as enhanced infectivity and seriousness, and achieve the effects of simple and fast operation, easy analysis, and simple and objective interpretation of results

CN113308571APending Publication Date: 2021-08-27内蒙古自治区国际蒙医医院 +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2021-08-27

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Abstract

The invention relates to a reagent and a detection method for detecting a D614G mutant of an SARS-COV-2 virus. The reagent comprises a primer group, a probe, a negative reference substance and a positive reference substance, the primer group comprises a forward primer 3037-F and a reverse primer 3037-R containing a mutation sequence of a 3037 site of the SARS-CoV-2 virus, and a forward primer 23403-F and a reverse primer 23403-R containing a mutation sequence of a 23403 of the SARS-CoV-2 virus, probes comprise a probe 3037-probe for identifying a mutation sequence of a site 3037 of the SARS-CoV-2 virus and a probe 23403-probe for identifying a mutation sequence of a site 23403 of the SARS-CoV-2 virus, the negative reference substance adopts sterilized ultrapure water, and the positive reference substance is a mixed solution of two DNA fragments of a fragment 3037-TEM containing the mutation sequence of the site 3037 and a fragment 23403-TEM containing the mutation sequence of the site 23403.
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Description

technical field

[0001] The invention relates to the field of genetic detection, in particular to a reagent for detecting the D614G mutant of SARS-COV-2 virus and a detection method thereof. Background technique

[0002] Since the outbreak of the new coronavirus (SARS-CoV-2), the number of new coronary pneumonia infections in more than 200 countries around the world has continued to rise. As of April 8, 2021, the new coronavirus has infected more than 1.3 B people, and the cumulative death toll has exceeded 280. Ten thousand.

[0003] The novel coronavirus is a single-stranded positive-sense RNA virus belonging to the family Coronaviridae and the subgenus Sarbecovirus in the genus Betacoronavirus. Its genome contains 11 genes, of which 5 genes with more important functions are: ORF1ab gene encodes a multifunctional protein involved in viral RNA transcription and replication, including protease responsible for cutting polyproteins; S gene encodes spike protein S, and its Inf...

Examples

Embodiment 1

[0041] Embodiment one, by figure 1 Provided, the present invention provides a reagent for detecting the D614G mutant of SARS-CoV-2 virus, including primer set, probe, negative control substance and positive control substance, and the primer set includes 3037 sites of SARS-CoV-2 virus Forward primer 3037-F and reverse primer 3037-R of the mutated sequence and forward primer 23403-F and reverse primer 23403-R in the mutated sequence of SARS-CoV-2 virus 23403, the probes include SARS-CoV -2 The probe 3037-probe of the 3037-site mutation sequence of the virus and the probe 23403-probe for recognizing the 23403-site mutation sequence of the SARS-CoV-2 virus, the negative control substance uses sterilized ultrapure water, and the positive control substance contains 3037 A mixture of two kinds of DNA fragments, the fragment 3037-TEM of the site mutation sequence and the fragment 23403-TEM containing the 23403 mutation sequence.

[0042] The sequence of the forward primer 3037-F is 5...