Torrent bioreactor for cell culture

Abstract

The invention relates to the technical field of cell culture equipment, and discloses a torrent bioreactor for cell culture. The torrent bioreactor comprises a vibrator, a tank body and a controller, wherein the tank body is mounted above the vibrator, the vibrator is electrically connected with the controller, the inner cavity of the tank body is filled with a culture solution, a sealing cover is mounted at the top of the tank body, a vertical mixing assembly for mixing the culture solution is arranged on the outer wall of the vibrator, and a collector is arranged on the bottom surface of the sealing cover. Flowing liquid beads are placed in the culture solution in the tank body, and the flowing liquid beads are promoted to move up and down in the culture solution by utilizing attraction force of a lower electromagnet and an upper electromagnet which are arranged in an up-down staggered manner to the flowing liquid beads, so that the flowing liquid beads flow in a smooth curve form under the action of gravity, centripetal force and magnetic acting force. The soft characteristic of the flowing liquid beads is matched, so that the effect of mixing the culture solution up and down is improved on the premise of avoiding increasing the shear force on the culture solution.

Description

technical field [0001] The invention relates to the technical field of cell culture equipment, in particular to a torrent bioreactor for cell culture. Background technique [0002] The torrent bioreactor is based on a new type of oxygen transfer mechanism without bubbling. The torrent is mechanically shaken by the torrent oscillator to generate a torrent, so that the culture medium repeatedly flushes the oxygen molecule layer on the inner surface of the cell culture bag, and then quickly dissolves the oxygen molecules in the culture medium. , to meet the needs of normal production and metabolism of cells. In the torrent bioreactor, the bottom of the tank is conical and fixed to the surface of the shaker. When the reactor is working, with the rotation of the shaker, the liquid in the reactor forms a vortex column, which expands the gas-liquid exchange area, and most of the The medium is on one side of the inner wall of the tank, and a liquid film is formed on the other side....

AI Technical Summary

Problems solved by technology

[0005] In view of the deficiencies in the use of the existing torrent bioreactor proposed in the background technology, the present invention provides a torrent bioreactor for cell culture, which has the ability to improve the mixing effect of the defoamer and improve the defoamer's defoaming effect. Foam ability and the advantages of improving the vertical mixing degree of the culture solution solve the problems raised in the above-mentioned background technology

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