Rotary cell resuscitation instrument

Abstract

The invention relates to a rotary cell resuscitation instrument. The rotary cell resuscitation instrument comprises a machine body, a water bath kettle and a water bath execution device, wherein the water bath execution device comprises a lifting machine head and a cryopreservation tube bearing device on the lifting machine head; the cryopreservation tube bearing device comprises an immersion bath tray with spacer bushes, a cryopreservation tube fixing mechanism and a rotating mechanism; the immersion bath tray is mounted on a hollow rotating shaft of the rotating mechanism; a tray seat on a disc-shaped cryopreservation tube shelf of the cryopreservation tube fixing mechanism can axially slide outside the hollow rotating shaft; a driving head which axially slides and is in circumferential linkage is arranged in the hollow rotating shaft; a connecting pin on the driving head penetrates through an avoiding slotted hole in the hollow rotating shaft and then is connected with the tray seat; the driving head is connected with a driving mandrel; a cryopreservation tube positioning disc of the disc-shaped cryopreservation tube shelf is detachably assembled on the tray seat in a pushing and inserting manner along a U-shaped opening; cryopreservation tube shelf seat holes in the cryopreservation tube positioning disc are in one-to-one correspondence to the spacer bushes and are in sealing fit with the spacer bushes; and a disc-shaped cover plate which is used for opening and closing upper ports of the cryopreservation tube shelf seat holes and elastically pressing cryopreservation tubes is arranged on the tray seat. The rotary cell resuscitation instrument has the advantages of high cell resuscitation efficiency, low pollution and convenience in operation.

Description

technical field [0001] The present invention relates to biological experiment equipment, specifically equipment for cell recovery. Background technique [0002] The main experimental objects of cell biology research are various primary cultured or subcultured cells or cell lines. In order to avoid the pollution caused by the cultured cells in the long-term in vitro culture process or the gene mutation caused by multiple passages, the experimenter will freeze the cultured cells during the vigorous growth period, and store the frozen cells in a container with cell freezing solution The cells are frozen in tubes and then placed in liquid nitrogen. In this ultra-low temperature environment, the cells can be stored for a long time. When the experiment is needed, the experimenter can resuscitate the frozen cells in left and right warm water for the required experiments, which can ensure the genetic stability of the cells to the greatest extent. [0003] In existing biological ex...

AI Technical Summary

Problems solved by technology

However, it adopts the method of directly immersing in a water bath; there is still the problem of low cell recovery efficiency, and if the shaking method is considered, the spacer will inevitably be increased, and the cryopreservation tube will be submerged in the spacer, resulting in inconvenient handling of the cryopreservation tube

And the cryopreservation tube is not fixed in the spacer, and the way of shaking can easily cause the cryopreservation tube to move in the spacer; and if the spacer and the cryopreservation tube are closely matched, it will further make it inconvenient to take and put the cryopreservation tube

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