Method for cloning embryos by batch fusion of magnetic donor cells

A technique for donor cells and cloned embryos, applied in the biological field, can solve the problems of cumbersome operation, long time, and low fusion efficiency, and achieve the effects of convenient operation, guaranteed accuracy, and reduced in vitro operation time

Active Publication Date: 2021-09-07
INST OF ANIMAL SCI & VETERINARY HUBEI ACADEMY OF AGRI SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The technical problem solved by the present invention is to overcome the defects of manual dialing, long time, cumbersome operation and low fusion efficiency in the prior art, and provide a method for batch fusion of cloned embryos using magnetic donor cells

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  • Method for cloning embryos by batch fusion of magnetic donor cells
  • Method for cloning embryos by batch fusion of magnetic donor cells
  • Method for cloning embryos by batch fusion of magnetic donor cells

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Embodiment Construction

[0027] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0028] see Figure 1-3 , the present invention provides a technical solution: a method for batch fusion of cloned embryos using donor cells with a magnetic field, comprising the following steps,

[0029] S1: Acquisition of oocytes and somatic cells;

[0030] S2: In vitro maturation culture of oocytes, with pre-warmed maturation medium containing 10% PFF (follicular fluid), 0.5 μg / mL FSH (follicle-stimulating hormone), 0.5 μg / mL LH (luteinizing hormone), 10 ng...

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Abstract

The invention discloses a method for cloning embryos by batch fusion of magnetic donor cells, which changes a traditional fusion method. An electrified solenoid coil is added to an electrode on any side, so that the fusion electrode has strong magnetism to attract magnetic donor cells to be adhered to the electrode, and formed electrode-donor cell-oocyte contact surfaces are on the same vertical plane, so that seamless attachment is realized, the accuracy of electric shock is ensured, the in-vitro operation time is shortened, and the operation is convenient. According to the method, the fusion time for cloning embryos is increased from 3-5 seconds per embryo to 3-5 seconds per 20-30 embryos, the in-vitro operation time for cloning embryos is greatly shortened, the fusion efficiency is improved, and technical support is provided for batch production of cloned embryos in a laboratory. The method has the advantages of convenience and rapidness in operation and high fusion efficiency.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for batch fusion of cloned embryos by using magnetic donor cells. Background technique [0002] Somatic cell cloning technology refers to the suppression of culture of animal somatic cells, so that the cells are in a dormant state. Using the method of nuclear transfer, using cell disassembly or cell recombination technology, enucleated oocytes are used as nuclear acceptors, somatic cells or nuclei with a small amount of cytoplasm, namely nucleoplastids, are used as nuclear donors, and the latter is transferred into the former. , to construct a recombinant embryo, the donor nucleus is reprogrammed in the cytoplasm of the enucleated oocyte, and the cleavage is started, the embryonic development process is started, the technology of gestation and birth, and the cloning of animals can also be called somatic cell nuclear transfer technology . [0003] Somatic cell cloning techn...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/877C12N13/00C12N5/075C12N5/10
CPCC12N15/877C12N13/00C12N5/0609C12N5/0686C12N2501/11C12N2501/31C12N2500/25C12N2510/00
Inventor 华再东郭帅朱向星王肆玖周昌胜
Owner INST OF ANIMAL SCI & VETERINARY HUBEI ACADEMY OF AGRI SCI
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