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Duck mitochondrion COI gene amplification primer and application thereof

A gene amplification and mitochondrial technology, applied in the field of duck mitochondrial COI gene amplification primers, to achieve the effect of single band and high amplification efficiency

Pending Publication Date: 2021-10-08
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no widely used universal primer with good specificity for the duck COI gene

Method used

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  • Duck mitochondrion COI gene amplification primer and application thereof
  • Duck mitochondrion COI gene amplification primer and application thereof
  • Duck mitochondrion COI gene amplification primer and application thereof

Examples

Experimental program
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Embodiment Construction

[0030] The application will be described in further detail below in conjunction with the accompanying drawings. It is necessary to point out that the following specific embodiments are only used to further illustrate the application, and cannot be interpreted as limiting the protection scope of the application. The above application content makes some non-essential improvements and adjustments to this application.

[0031] 1. Materials

[0032] The methods used in this example are conventional methods known to those skilled in the art unless otherwise specified, and the reagents and other materials used are all commercially available products unless otherwise specified.

[0033] 2. Method

[0034] Design and synthesis of duck mitochondrial COI (cytochrome oxidase subunit I (cytochrome oxidase subunit I)) gene amplification primers and complete the amplification of duck mitochondrial COI gene are carried out through the following steps.

[0035] 2.1 Download sequence

[0036...

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Abstract

The invention relates to the technical field of duck biological mitochondrion gene acquisition, and particularly discloses a duck mitochondrion COI gene amplification primer and application thereof. The sequence of the primer is as follows: the sequence of an upstream primer COI-F: 5'-CCATCTTACCCGTGACCT-3', and the sequence of a downstream primer COI-R: 5'-TGGCTTGAAACCAGTGTA-3'. The duck mitochondrion COI gene amplification primer can increase the length of an obtained duck mitochondrion COI gene segment to about 1551bp, has the characteristics of single band and high amplification efficiency, and can better meet the requirements of subsequent duck mitochondrion genome research and duck population genetic diversity evaluation.

Description

technical field [0001] The invention belongs to the technical field of duck biological mitochondrial gene acquisition, and in particular relates to a duck mitochondrial COI gene amplification primer and application thereof. Background technique [0002] Meat duck industry is an important part of my country's animal husbandry. Duck is the third largest meat industry after pork and chicken in my country. In 2020, the number of meat ducks sold in my country will reach 4.68 billion, accounting for more than 80% of the global total. The output of meat ducks will exceed 10.5 million tons, and the output value will exceed 125 billion yuan, indicating that my country's demand for duck meat is on the rise. In addition, ducks also play an important role in sociocultural and biomedical research. Endemic duck breeds are a valuable genetic resource, and their high adaptability to the local environment enables them to survive well. [0003] Endemic duck species have great genetic diver...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/6888C12Q1/686
CPCC12Q1/6888C12Q1/686C12Q2565/125C12Q2535/101C12Q1/6876C12Q2600/124
Inventor 金四华贾羽晴耿照玉程智中税斐片慧芳江洪峰夏晶晶郑书丽高伟凤
Owner ANHUI AGRICULTURAL UNIVERSITY
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